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- W2810921664 endingPage "3110" @default.
- W2810921664 startingPage "3093" @default.
- W2810921664 abstract "It has long been suggested that chromatin may form a fiber with a diameter of ~ 30 nm that suppresses transcription. Despite nearly four decades of study, the structural nature of the 30-nm chromatin fiber and conclusive evidence of its existence in vivo remain elusive. The key support for the existence of specific 30-nm chromatin fiber structures is based on the determination of the structures of reconstituted nucleosome arrays using X-ray crystallography and single-particle cryo-electron microscopy coupled with glutaraldehyde chemical cross-linking. Here we report the characterization of these nucleosome arrays in solution using analytical ultracentrifugation, NMR, and small-angle X-ray scattering. We found that the physical properties of these nucleosome arrays in solution are not consistent with formation of just a few discrete structures of nucleosome arrays. In addition, we obtained a crystal of the nucleosome in complex with the globular domain of linker histone H5 that shows a new form of nucleosome packing and suggests a plausible alternative compact conformation for nucleosome arrays. Taken together, our results challenge the key evidence for the existence of a limited number of structures of reconstituted nucleosome arrays in solution by revealing that the reconstituted nucleosome arrays are actually best described as an ensemble of various conformations with a zigzagged arrangement of nucleosomes. Our finding has implications for understanding the structure and function of chromatin in vivo." @default.
- W2810921664 created "2018-07-10" @default.
- W2810921664 creator A5011813946 @default.
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- W2810921664 creator A5074374757 @default.
- W2810921664 creator A5075228460 @default.
- W2810921664 date "2018-09-01" @default.
- W2810921664 modified "2023-10-17" @default.
- W2810921664 title "Revisit of Reconstituted 30-nm Nucleosome Arrays Reveals an Ensemble of Dynamic Structures" @default.
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