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• W2810939097 abstract "In this paper, we demonstrate the application of electrical cell-substrate impedance sensing (ECIS) technology for measuring differences in the formation of a strong and durable endothelial barrier model. In addition, we highlight the capacity of ECIS technology to model the parameters of the physical barrier associated with (I) the paracellular space (referred to as Rb) and (II) the basal adhesion of the endothelial cells (α, alpha). Physiologically, both parameters are very important for the correct formation of endothelial barriers. ECIS technology is the only commercially available technology that can measure and model these parameters independently of each other, which is important in the context of ascertaining whether a change in overall barrier resistance (R) occurs because of molecular changes in the paracellular junctional molecules or changes in the basal adhesion molecules. Finally, we show that the temporal changes observed in the paracellular Rb can be associated with changes in specific junctional proteins (CD144, ZO-1, and catenins), which have major roles in governing the overall strength of the junctional communication between neighbouring endothelial cells." @default.
• W2810939097 created "2018-07-10" @default.
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• W2810939097 date "2018-07-04" @default.
• W2810939097 modified "2023-10-18" @default.
• W2810939097 title "The Importance of Multifrequency Impedance Sensing of Endothelial Barrier Formation Using ECIS Technology for the Generation of a Strong and Durable Paracellular Barrier" @default.
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• W2810939097 doi "https://doi.org/10.3390/bios8030064" @default.
• W2810939097 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6163417" @default.
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