FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2810986398> ?p ?o ?g. }

• W2810986398 endingPage "1900" @default.
• W2810986398 startingPage "1891" @default.
• W2810986398 abstract "Universal screening for Lynch syndrome in colorectal cancer is recommended, and immunohistochemistry for the mismatch repair proteins is commonly used. To reduce cost, some screen using only MSH6 and PMS2, with reflex to the partner stain if either are absent (two-stain method). An expression pattern revealing absent MSH2 and intact MSH6 is not expected, but could result in failed Lynch syndrome detection. We analyzed tumors with absent MSH2 but any degree of MSH6 expression to determine if the two-stain method could miss MSH2 mutations. One-thousand seven-hundred thirty colorectal cancer patients from the Ohio Colorectal Cancer Prevention Initiative underwent tumor screening using microsatellite instability and immunohistochemistry. The two-stain method was used for 1235 cases; staining for all four proteins was completed for 495 cases. The proportion of positive cells and staining intensity were reviewed for MSH6, as well as MSH2 when available. Patients with mismatch repair deficiency underwent next-generation sequencing of germline DNA for mismatch repair genes. If negative, tumor next-generation sequencing was performed to assess for somatic mutations. Overall, thirty-three (1.9%, 33/1730) MSH2-absent cases were identified. Of those, fourteen had no MSH6 expression but eight (0.5%, 8/1730) had ambiguous and eleven (0.6%, 11/1730) had convincing MSH6 expression that could have been interpreted as intact. Germline next-generation sequencing identified MSH2 mutations in 11/14 cases with absence of both stains, 7/8 cases with ambiguous MSH6 expression, and 9/11 cases with convincing MSH6 expression. All remaining cases, except one, had double somatic mutations. The two-stain method fails to detect some patients with Lynch syndrome: (1) significant staining weaker than the control may be incorrectly interpreted as intact MSH6, or (2) Weak or focal/patchy MSH6 can be retained with the absence of MSH2. Accordingly, we recommend the four-stain method be used for optimal Lynch syndrome screening detection." @default.
• W2810986398 created "2018-07-10" @default.
• W2810986398 creator A5012798070 @default.
• W2810986398 creator A5017541508 @default.
• W2810986398 creator A5018882849 @default.
• W2810986398 creator A5054707329 @default.
• W2810986398 creator A5056973866 @default.
• W2810986398 creator A5061124226 @default.
• W2810986398 creator A5064385855 @default.
• W2810986398 creator A5067713698 @default.
• W2810986398 date "2018-12-01" @default.
• W2810986398 modified "2023-09-30" @default.
• W2810986398 title "Two-stain immunohistochemical screening for Lynch syndrome in colorectal cancer may fail to detect mismatch repair deficiency" @default.
• W2810986398 cites W1207462543 @default.
• W2810986398 cites W1534073104 @default.
• W2810986398 cites W1758789194 @default.
• W2810986398 cites W1940241680 @default.
• W2810986398 cites W1968028641 @default.
• W2810986398 cites W1986937402 @default.
• W2810986398 cites W1988790156 @default.
• W2810986398 cites W2001538359 @default.
• W2810986398 cites W2004090060 @default.
• W2810986398 cites W2032465462 @default.
• W2810986398 cites W2039808953 @default.
• W2810986398 cites W2044386531 @default.
• W2810986398 cites W2055590446 @default.
• W2810986398 cites W2058438744 @default.
• W2810986398 cites W2062850641 @default.
• W2810986398 cites W2064329151 @default.
• W2810986398 cites W2083097481 @default.
• W2810986398 cites W2095878672 @default.
• W2810986398 cites W2097502584 @default.
• W2810986398 cites W2102826981 @default.
• W2810986398 cites W2104818405 @default.
• W2810986398 cites W2112565258 @default.
• W2810986398 cites W2116138166 @default.
• W2810986398 cites W2121429001 @default.
• W2810986398 cites W2145297551 @default.
• W2810986398 cites W2145494417 @default.
• W2810986398 cites W2147274538 @default.
• W2810986398 cites W2165606389 @default.
• W2810986398 cites W2165736459 @default.
• W2810986398 cites W2410025648 @default.
• W2810986398 cites W2565479307 @default.
• W2810986398 cites W54029553 @default.
• W2810986398 doi "https://doi.org/10.1038/s41379-018-0058-y" @default.
• W2810986398 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6800091" @default.
• W2810986398 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29967423" @default.
• W2810986398 hasPublicationYear "2018" @default.
• W2810986398 type Work @default.
• W2810986398 sameAs 2810986398 @default.
• W2810986398 citedByCount "49" @default.
• W2810986398 countsByYear W28109863982019 @default.
• W2810986398 countsByYear W28109863982020 @default.
• W2810986398 countsByYear W28109863982021 @default.
• W2810986398 countsByYear W28109863982022 @default.
• W2810986398 countsByYear W28109863982023 @default.
• W2810986398 crossrefType "journal-article" @default.
• W2810986398 hasAuthorship W2810986398A5012798070 @default.
• W2810986398 hasAuthorship W2810986398A5017541508 @default.
• W2810986398 hasAuthorship W2810986398A5018882849 @default.
• W2810986398 hasAuthorship W2810986398A5054707329 @default.
• W2810986398 hasAuthorship W2810986398A5056973866 @default.
• W2810986398 hasAuthorship W2810986398A5061124226 @default.
• W2810986398 hasAuthorship W2810986398A5064385855 @default.
• W2810986398 hasAuthorship W2810986398A5067713698 @default.
• W2810986398 hasBestOaLocation W28109863981 @default.
• W2810986398 hasConcept C104317684 @default.
• W2810986398 hasConcept C111425858 @default.
• W2810986398 hasConcept C121608353 @default.
• W2810986398 hasConcept C126322002 @default.
• W2810986398 hasConcept C13514818 @default.
• W2810986398 hasConcept C142724271 @default.
• W2810986398 hasConcept C180754005 @default.
• W2810986398 hasConcept C204232928 @default.
• W2810986398 hasConcept C2776559941 @default.
• W2810986398 hasConcept C2776674815 @default.
• W2810986398 hasConcept C2778677574 @default.
• W2810986398 hasConcept C2779115348 @default.
• W2810986398 hasConcept C2779767149 @default.
• W2810986398 hasConcept C2781294515 @default.
• W2810986398 hasConcept C501734568 @default.
• W2810986398 hasConcept C526805850 @default.
• W2810986398 hasConcept C54355233 @default.
• W2810986398 hasConcept C60748783 @default.
• W2810986398 hasConcept C61320498 @default.
• W2810986398 hasConcept C71924100 @default.
• W2810986398 hasConcept C74864618 @default.
• W2810986398 hasConcept C86803240 @default.
• W2810986398 hasConceptScore W2810986398C104317684 @default.
• W2810986398 hasConceptScore W2810986398C111425858 @default.
• W2810986398 hasConceptScore W2810986398C121608353 @default.
• W2810986398 hasConceptScore W2810986398C126322002 @default.
• W2810986398 hasConceptScore W2810986398C13514818 @default.
• W2810986398 hasConceptScore W2810986398C142724271 @default.
• W2810986398 hasConceptScore W2810986398C180754005 @default.
• W2810986398 hasConceptScore W2810986398C204232928 @default.
• W2810986398 hasConceptScore W2810986398C2776559941 @default.

• next