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• W2811280176 abstract "The isolation and sorting of cells is an important process in research and hospital labs. Most large research and commercial labs incorporate fluorescently or magnetically labeled antibodies adherent to cell surface antigens for cell identification and separation. In this paper, a process is described that merges biochemical labeling with ultrasound-based separation. Instead of lasers and fluorophore tags, or magnets and magnetic particle tags, the technique uses ultrasound and microbubble tags. Streptavidin-labeled microbubbles were mixed with a human acute lymphoblastic leukemia cell line, CCL 119, conjugated with biotinylated anti-CD7 antibodies. Tagged cells were forced under ultrasound, and their displacement and velocity quantified. Differential displacement in a flow stream was quantified against erythrocytes, which showed almost no displacement under ultrasound. A model for the acoustic radiation force on the conjugated pairs compares favorably with observations. This technology may improve on current time-consuming and costly purification procedures." @default.
• W2811280176 created "2018-07-10" @default.
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• W2811280176 date "2018-07-01" @default.
• W2811280176 modified "2023-09-30" @default.
• W2811280176 title "Ultrasound-based cell sorting with microbubbles: A feasibility study" @default.
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• W2811280176 doi "https://doi.org/10.1121/1.5044405" @default.
• W2811280176 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6029934" @default.
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