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• W2811332371 abstract "Marijuana, a drug derived from the Cannabis sativa L. plant, is the world's most consumed illicit drug. In this paper, a total of 156 marijuana samples seized in the state of Espírito Santo (ES), Brazil were studied and analysed by proton nuclear magnetic resonance (1H NMR) spectroscopy to identify the major cannabinoids present. A crude extract of all samples was purified using high performance liquid chromatography so that these compounds could serve as reference substances. Nine fractions were obtained and analysed by 1H NMR and gas chromatography–mass spectrometry (GC–MS), with five presented cannabinoids. ∆9-THC (Δ9-trans-tetrahydrocannabinol), ∆9-THCA (∆9-tetrahydrocannabinolic acid), ∆8-THC (∆8-tetrahydrocannabinol), 11-hydroxycannabinol, CBV (cannabivarin), and CBN (cannabinol) were found, and their chemical structures were confirmed by GC–MS. The latter compound was obtained with high purity (≈100%), while the others were obtained as less complex mixtures with purity higher than 75% (except for Δ8-THC). Principal component analysis (PCA) was used on the 1H NMR spectra of the 156 samples, and it was found that the samples were grouped according to the months, differentiating into two groups (from July 2014 to January 2015 and from February 2015 to July 2015), where non-grouping was observed from four macro-regions of the ES state (North, Central, Metropolitan, and South). The chemical profile of the seized samples was correlated to the 1H NMR spectrum of an isolated CBN sub-fraction, in which the group formed by samples seized in the year 2015 presented lower CBN content in the chemical composition. From the PCA score plot, two groups of samples were confirmed using the partial least squares discriminant analysis and orthogonal projections to latent structures classification methods." @default.
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• W2811332371 date "2018-09-01" @default.
• W2811332371 modified "2023-10-15" @default.
• W2811332371 title "Extraction and isolation of cannabinoids from marijuana seizures and characterization by 1H NMR allied to chemometric tools" @default.
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• W2811332371 doi "https://doi.org/10.1016/j.scijus.2018.06.005" @default.
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