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• W2811441310 abstract "Paired-end RNA sequencing (RNA-seq) is usually applied to the quantification of long transcripts such as messenger or long non-coding RNAs, in which case overlapping pairs are discarded. In contrast, RNA-seq on short RNAs (≤ 200 nt) is typically carried out in single-end mode, as the additional cost associated with paired-end would only translate into redundant sequence information. Here, we exploit paired-end sequencing of short RNAs as a strategy to filter out sequencing errors and apply this method to the identification of adenosine-to-inosine (A-to-I) RNA editing events on human precursor microRNA (pre-miRNA) and mature miRNA. Combined with RNA immunoprecipitation sequencing (RIP-seq) of A-to-I RNA editing enzymes, this method takes full advantage of deep sequencing technology to identify RNA editing sites with unprecedented resolution in terms of editing efficiency." @default.
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• W2811441310 date "2018-01-01" @default.
• W2811441310 modified "2023-10-02" @default.
• W2811441310 title "High-Quality Overlapping Paired-End Reads for the Detection of A-to-I Editing on Small RNA" @default.
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• W2811441310 doi "https://doi.org/10.1007/978-1-4939-8624-8_13" @default.
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