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• W281164337 abstract "Objective To establish a system for isolation and culture of bone marrow mesenchymal stem cells (MSCs) from Kun-ming mouse in intro and to identify the characteristics of the cells,directional neuronal and adipocytic induction after culture expansion. MethodMSCs were isolated and cultivated from the bone marrow of Kun-ming mice by investigating their adherence-dependent growth characters. The morphological characters of MSCs were observed under inverted phase contrast microscope. Shape characteristics of MSCs were studied by AKP staining,Hoechst33258 staining,white Giemsa staining. MSCs were induced into neurone-like cells by β-ME,and stained with H.E after induction,then their characteristics were observed under inverted phase contrast microscope. The differentiation ratio analysis of differentiated neurocytes was detected by immunocyte-fluorescence of NF-200 and NSE. MSCs were induced into adipocytes by horse serum,the process of growth in vitro,was observed and the differentiation ratio analysis of differentiated adipocytes was detected by Oil Red O stain. Result Separated MSCs were fibroblast-like cells,they had several nucleoluses,and the positive rates of AKP staining were 93.2%±1.5%. MSCs could differentiate into several phenotype characteristic neurocytes after induced by β-ME,their shape characteristics were similar with primer passage of mouse full-brain cells. Both NF-200 and NSE immunocyte-fluorescence of Neurones after induction were positive,and the positive rates of NF-200 and NSE were 85.4%±1.8% and 82.7%±2.1%,respectively. MSCs could differentiate into adipocytes in 100 percent after induced by horse serum,and Oil Red O stain was positive.ConclusionMesenchymal stem cells of Kun-ming mouse can be successfully isolated and cultivated from mouse bone marrow by keeping its adherence-dependent growth characters. They have directional trans-differentiation potentiality to various neurocytes and adipocytes." @default.
• W281164337 created "2016-06-24" @default.
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• W281164337 date "2008-01-01" @default.
• W281164337 modified "2023-09-22" @default.
• W281164337 title "Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells into Neurocytes and Adipocytes Induced in vitro" @default.
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• W281164337 hasPublicationYear "2008" @default.
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