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• W2816851 abstract "The Cys loop receptors, a family of ligand-gated ion channels, mediate fast synaptic transmission throughout the peripheral and central nervous systems. These are large multisubunit proteins, whose primary function is to transduce a chemical signal, binding of a neurotransmitter, into an electrical signal, ion flux across the cell membrane. These receptors have been implicated in several disease states and represent major therapeutic targets. The work presented in this thesis focuses on the chemical-scale elucidation of Cys loop receptors. The main approach of this work is the structure-function study using in vivo nonsense suppression methods. This technique allows for the site-specific incorporation of an unnatural amino acid into a protein expressed in a living cell.Nonsense suppression methods were used to incorporate a series of fluorinated tryptophan derivatives into the binding site of the 5-HT3R. This study identified a cation-pi interaction between Trp 183 and the neurotransmitter, serotonin. A similar study using fluorinated phenylalanine derivatives identified a cation-pi binding site at Tyr 198 in the GABAC receptor. These studies build on previous work from our research group and provide further evidence that the cation-pi interaction is a common feature in ligand recognition by Cys loop receptors.Nonsense suppression was also used to examine the role of several tyrosine residues in the 5-HT3R. Here the findings demonstrated that the side chains of Tyr 143 and 153 make functionally important hydrogen bonds. These data were used to refine several computational models of serotonin docked into the binding site.Structure-function studies of two conserved prolines in the M2-M3 loop showed that this region of the receptor is involved in the conformational changes associated with receptor activation. The data also provide preliminary evidence that Pro 308 may serve as hinge for the gating movement of the M2 helix." @default.
• W2816851 created "2016-06-24" @default.
• W2816851 creator A5019462530 @default.
• W2816851 date "2005-01-01" @default.
• W2816851 modified "2023-09-27" @default.
• W2816851 title "Chemical scale investigations of ligand-gated ion channels using unnatural amino acids" @default.
• W2816851 cites W105839754 @default.
• W2816851 cites W1497011462 @default.
• W2816851 cites W1504812383 @default.
• W2816851 cites W1550469113 @default.
• W2816851 cites W1564990428 @default.
• W2816851 cites W1571140265 @default.
• W2816851 cites W1591881216 @default.
• W2816851 cites W1603646817 @default.
• W2816851 cites W1615624892 @default.
• W2816851 cites W1616226939 @default.
• W2816851 cites W1788430178 @default.
• W2816851 cites W1922786159 @default.
• W2816851 cites W1923513268 @default.
• W2816851 cites W1966264250 @default.
• W2816851 cites W1968497607 @default.
• W2816851 cites W1969154943 @default.
• W2816851 cites W1969787762 @default.
• W2816851 cites W1970268881 @default.
• W2816851 cites W1971422019 @default.
• W2816851 cites W1971503114 @default.
• W2816851 cites W1973111841 @default.
• W2816851 cites W1975360788 @default.
• W2816851 cites W1975744163 @default.
• W2816851 cites W1976141244 @default.
• W2816851 cites W1977262106 @default.
• W2816851 cites W1977896619 @default.
• W2816851 cites W1978162314 @default.
• W2816851 cites W1978913982 @default.
• W2816851 cites W1978922710 @default.
• W2816851 cites W1979001025 @default.
• W2816851 cites W1981838079 @default.
• W2816851 cites W1983234151 @default.
• W2816851 cites W1984302804 @default.
• W2816851 cites W1985926016 @default.
• W2816851 cites W1988367902 @default.
• W2816851 cites W1988867706 @default.
• W2816851 cites W1990915431 @default.
• W2816851 cites W1994397958 @default.
• W2816851 cites W1996083272 @default.
• W2816851 cites W1997875114 @default.
• W2816851 cites W2001801891 @default.
• W2816851 cites W2003245313 @default.
• W2816851 cites W2004368544 @default.
• W2816851 cites W2004976660 @default.
• W2816851 cites W2005843451 @default.
• W2816851 cites W2007724600 @default.
• W2816851 cites W201108989 @default.
• W2816851 cites W2011691554 @default.
• W2816851 cites W2013034672 @default.
• W2816851 cites W2015090760 @default.
• W2816851 cites W2015760510 @default.
• W2816851 cites W2018937595 @default.
• W2816851 cites W2022788156 @default.
• W2816851 cites W2023437282 @default.
• W2816851 cites W2024316065 @default.
• W2816851 cites W2024463726 @default.
• W2816851 cites W2027217660 @default.
• W2816851 cites W2027886505 @default.
• W2816851 cites W2029185288 @default.
• W2816851 cites W2032493626 @default.
• W2816851 cites W2036931150 @default.
• W2816851 cites W2038114967 @default.
• W2816851 cites W2038522084 @default.
• W2816851 cites W2046755082 @default.
• W2816851 cites W2047572594 @default.
• W2816851 cites W2050251045 @default.
• W2816851 cites W2050352579 @default.
• W2816851 cites W2051852738 @default.
• W2816851 cites W2052521026 @default.
• W2816851 cites W2054355850 @default.
• W2816851 cites W2056305953 @default.
• W2816851 cites W2057451487 @default.
• W2816851 cites W2058090336 @default.
• W2816851 cites W2059971190 @default.
• W2816851 cites W2061112696 @default.
• W2816851 cites W2061957367 @default.
• W2816851 cites W2062162317 @default.
• W2816851 cites W2064716344 @default.
• W2816851 cites W2065236803 @default.
• W2816851 cites W2065599864 @default.
• W2816851 cites W2067013502 @default.
• W2816851 cites W2067219399 @default.
• W2816851 cites W2070412797 @default.
• W2816851 cites W2071906211 @default.
• W2816851 cites W2072131303 @default.
• W2816851 cites W2073286007 @default.
• W2816851 cites W2073640163 @default.
• W2816851 cites W2073990481 @default.
• W2816851 cites W2074808135 @default.
• W2816851 cites W2075276440 @default.
• W2816851 cites W2077456115 @default.
• W2816851 cites W2078366765 @default.
• W2816851 cites W2078953408 @default.
• W2816851 cites W2079234445 @default.

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