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- W2819356720 abstract "HomePlant DiseaseVol. 102, No. 9First Report of Anthracnose Caused by Colletotrichum truncatum on Basella alba in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Anthracnose Caused by Colletotrichum truncatum on Basella alba in ChinaX. X. Yang, Y. Liu, L. Zhang, X. Q. Huang, W. X. Wu, and L. H. XueX. X. YangSearch for more papers by this author, Y. Liu†Corresponding author: Y. Liu; E-mail: E-mail Address: liuyongdr@163.comSearch for more papers by this author, L. ZhangSearch for more papers by this author, X. Q. HuangSearch for more papers by this author, W. X. WuSearch for more papers by this author, and L. H. Xuehttp://orcid.org/0000-0002-1158-2152Search for more papers by this authorAffiliationsAuthors and Affiliations X. X. Yang Y. Liu † L. Zhang X. Q. Huang W. X. Wu , Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu, 610000, China L. H. Xue , College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China. Published Online:12 Jul 2018https://doi.org/10.1094/PDIS-01-18-0038-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Basella alba L. is an annual herb found in tropical Asia and Africa where it is widely used as a leaf vegetable. It is rich in vitamins A and C, iron, and calcium (FAO 1988). In September 2017, lesions were observed on young and mature leaves of B. alba in Chongzhou County (30°63′ N, 103°67′ E), Sichuan Province, China. The necrotic lesions (up to 5 mm in diameter) were semicircular or irregular-shaped with desiccated and grayish centers surrounded by deep red borders. Symptomatic leaves later withered and finally died, reducing the economic and edible value of B. alba. To identify the causal agent of leaf spots, small segments of symptomatic tissues were surface sterilized by washing in 75% ethanol for 1 min and rinsed three times in sterile water. The tissue was placed on potato dextrose agar (PDA) at 25°C for isolation. A fungus was consistently isolated from symptomatic tissues on PDA medium. Fungal colonies grown on PDA plates changed from grayish to dark gray and covered with olive-gray acervuli. Conidia were one-celled, hyaline, falcate to nearly straight, and measured 17.6 to 21.5 × 2.97 to 3.70 μm. These morphological and cultural characteristics were consistent with the description of Colletotrichum spp. For further identification, genomic DNA was extracted from a single-spore isolate Ba-1 using Fungi DNA Kit (OMEGA Biotech, Guangzhou, China). PCR amplification and sequence analysis of six coding and noncoding regions of the ribosomal internal transcribed spacer (ITS) (MG604922), actin (ACT) (MG604923), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (MG604924), chitin synthase (CHS-1) (MG604925), β-tubulin 2 (TUB2) (MG604926), and calmodulin (CAL) (MG604927) genes (Damm et al. 2012) revealed 99 to 100% sequence identity with C. truncatum strains (GenBank accession nos. KX364059, KF488581, KC109579, KT778592, KC109476, and KF114851, respectively). Pathogenicity of isolate Ba-1 was confirmed by inoculating 18 young leaves of B. alba with a conidial suspension (5 × 106 conidia/ml) of Ba-1, with sterile distilled water as a control. All inoculated and control leaves were incubated in moist chambers at 25°C. Isolate Ba-1 caused symptoms similar to those in the field within 8 days, and the isolates of C. truncatum were recovered from the inoculated leaves. No symptoms were present on the leaves of plants treated with sterile distilled water. On the basis of morphological, cultural, pathogenicity, and molecular characteristics, the causal agent of leaf spots on B. alba was identified as C. truncatum. To our knowledge, anthracnose caused by C. capsici and C. gloeosporioides (G. cingulata) (Minter et al. 2001) on B. alba has been reported previously, but this is the first report of C. truncatum causing anthracnose on B. alba in China. This identification will help producers select the best management practices for this devastating disease.References:Damm, U., et al. 2012. Stud. Mycol. 73:37. https://doi.org/10.3114/sim0010 Crossref, ISI, Google ScholarFAO. 1988. Page 103 in: Traditional Food Plants: A Resource Book for Promoting the Exploitation and Consumption of Food Plants in Arid, Semi-arid and Sub-humid Lands of Eastern Africa. FAO Food and Nutrition Paper 42. FAO, Rome, Italy. Google ScholarMinter, D. W., et al. 2001. Fungi of the Caribbean: An Annotated Checklist. PDMS Publishing, London, U.K. Google ScholarFunding: Funding was provided by Excellent Paper Fund of Sichuan Academy of Agricultural Sciences (grant no. 2018LWJJ-004).DetailsFiguresLiterature CitedRelated Vol. 102, No. 9 September 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Aug 2018Published: 12 Jul 2018Accepted: 23 Mar 2018 Pages: 1853-1853 Information© 2018 The American Phytopathological SocietyFundingExcellent Paper Fund of Sichuan Academy of Agricultural SciencesGrant/Award Number: 2018LWJJ-004Cited byColletotrichum truncatum (soyabean anthracnose)CABI Compendium, Vol. CABI CompendiumColletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1" @default.
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- W2819356720 title "First Report of Anthracnose Caused by <i>Colletotrichum truncatum</i> on <i>Basella alba</i> in China" @default.
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