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- W2820208916 abstract "In the body, living tissues or organ modules consist of highly organized cells and extracellular matrices in a hierarchical manner, such as the structure of blood vessels and the radial structures of hepatic lobules. The creation of artificial three-dimensional tissues possessing specific structures and functions similar to natural tissues is a key challenge in tissue engineering. A simple bottom-up approach was developed, called the hierarchical cell manipulation technique, with a nanometer-sized ECM matrix consisting of fibronectin (FN) and gelatin (G) on cell surfaces. About 10 nm-thick FN/G ECM films on cell surfaces were coated successfully using a layer-by-layer coating methodology. Another methodology, the “collagen coating method,” fabricates micrometer-sized level ECM layers onto cell surfaces. Various micrometer- or millimeter-sized 3D constructs with lower cell densities were successfully produced. By using these two methods, cell distances in a two- or three-dimensional view can be controlled by different thicknesses of ECM layers on cell surfaces below a single cell level." @default.
- W2820208916 created "2018-07-19" @default.
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- W2820208916 date "2018-07-06" @default.
- W2820208916 modified "2023-10-16" @default.
- W2820208916 title "Construction of Three‐Dimensional Tissues with Capillary Networks by Coating of Nanometer‐ or Micrometer‐Sized Film on Cell Surfaces" @default.
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- W2820208916 doi "https://doi.org/10.1002/9781119390350.ch3" @default.
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