FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W28228725> ?p ?o ?g. }

• W28228725 endingPage "219" @default.
• W28228725 startingPage "189" @default.
• W28228725 abstract "Protocols are presented describing a unique in vitro injury model and how to culture and mature mouse, rat, and human astrocytes for its use. This injury model produces widespread injury and astrocyte reactivity that enable quantitative measurements of morphological, biochemical, and functional changes in rodent and human reactive astrocytes. To investigate structural and molecular mechanisms of reactivity in vitro, cultured astrocytes need to be purified and then in vitro matured to reach a highly differentiated state. This is achieved by culturing astrocytes on deformable collagen-coated membranes in the presence of adult-derived horse serum (HS), followed by its stepwise withdrawal. These in vitro matured, process-bearing, quiescent astrocytes are then subjected to mechanical stretch injury by an abrupt pressure pulse from a pressure control device that briefly deforms the culture well bottom. This inflicts a measured reproducible, widespread strain that induces reactivity and injury in rodent and human astrocytes. Cross-species comparisons are possible because mouse, rat, and human astrocytes are grown using essentially the same in vitro treatment regimen. Human astrocytes from fetal cerebral cortex are compared to those derived from cortical biopsies of epilepsy patients (ages 1-12 years old), with regard to growth, purity, and differentiation. This opens a unique opportunity for future studies on glial biology, maturation, and pathology of human astrocytes. Prototypical astrocyte proteins including GFAP, S100, aquaporin4, glutamate transporters, and tenascin are expressed in mouse, rat, and human in vitro matured astrocyte. Upon pressure-stretching, rodent and human astrocytes undergo dynamic morphological, gene expression, and protein changes that are characteristic for trauma-induced reactive astrogliosis." @default.
• W28228725 created "2016-06-24" @default.
• W28228725 creator A5040042749 @default.
• W28228725 date "2011-11-11" @default.
• W28228725 modified "2023-09-23" @default.
• W28228725 title "An In Vitro Trauma Model to Study Rodent and Human Astrocyte Reactivity" @default.
• W28228725 cites W1502292420 @default.
• W28228725 cites W1607895415 @default.
• W28228725 cites W1827155399 @default.
• W28228725 cites W1966089167 @default.
• W28228725 cites W1967755760 @default.
• W28228725 cites W1969223713 @default.
• W28228725 cites W1970345181 @default.
• W28228725 cites W1971125401 @default.
• W28228725 cites W1972773032 @default.
• W28228725 cites W1973160402 @default.
• W28228725 cites W1976400860 @default.
• W28228725 cites W1977430640 @default.
• W28228725 cites W1979165828 @default.
• W28228725 cites W1979228875 @default.
• W28228725 cites W1980630988 @default.
• W28228725 cites W1982423786 @default.
• W28228725 cites W1983783671 @default.
• W28228725 cites W1988092837 @default.
• W28228725 cites W1989953010 @default.
• W28228725 cites W1995597143 @default.
• W28228725 cites W1997496613 @default.
• W28228725 cites W2004553739 @default.
• W28228725 cites W2006289803 @default.
• W28228725 cites W2009345585 @default.
• W28228725 cites W2010367054 @default.
• W28228725 cites W2015284998 @default.
• W28228725 cites W2015484726 @default.
• W28228725 cites W2017023169 @default.
• W28228725 cites W2019383843 @default.
• W28228725 cites W2019391998 @default.
• W28228725 cites W2019548178 @default.
• W28228725 cites W2019956654 @default.
• W28228725 cites W2022144987 @default.
• W28228725 cites W2024599421 @default.
• W28228725 cites W2024824899 @default.
• W28228725 cites W2030061210 @default.
• W28228725 cites W2034616897 @default.
• W28228725 cites W2039520731 @default.
• W28228725 cites W2044057452 @default.
• W28228725 cites W2047426662 @default.
• W28228725 cites W2048532873 @default.
• W28228725 cites W2052682099 @default.
• W28228725 cites W2053674306 @default.
• W28228725 cites W2055386145 @default.
• W28228725 cites W2057689655 @default.
• W28228725 cites W2061354480 @default.
• W28228725 cites W2063574557 @default.
• W28228725 cites W2063692247 @default.
• W28228725 cites W2064026116 @default.
• W28228725 cites W2067607447 @default.
• W28228725 cites W2069085932 @default.
• W28228725 cites W2069565315 @default.
• W28228725 cites W2074661552 @default.
• W28228725 cites W2082777494 @default.
• W28228725 cites W2082877682 @default.
• W28228725 cites W2082888555 @default.
• W28228725 cites W2085424855 @default.
• W28228725 cites W2093194302 @default.
• W28228725 cites W2094615027 @default.
• W28228725 cites W2094954632 @default.
• W28228725 cites W2097417059 @default.
• W28228725 cites W2116544803 @default.
• W28228725 cites W2117189609 @default.
• W28228725 cites W2117910176 @default.
• W28228725 cites W2118613169 @default.
• W28228725 cites W2122400789 @default.
• W28228725 cites W2124314024 @default.
• W28228725 cites W2127338217 @default.
• W28228725 cites W2129658871 @default.
• W28228725 cites W2136578373 @default.
• W28228725 cites W2138697863 @default.
• W28228725 cites W2142245128 @default.
• W28228725 cites W2144705128 @default.
• W28228725 cites W2145985401 @default.
• W28228725 cites W2152460583 @default.
• W28228725 cites W2155520053 @default.
• W28228725 cites W2160500827 @default.
• W28228725 cites W2166909073 @default.
• W28228725 cites W2399097270 @default.
• W28228725 cites W2410094973 @default.
• W28228725 cites W4241343595 @default.
• W28228725 cites W4244030872 @default.
• W28228725 cites W4294309388 @default.
• W28228725 cites W4300520946 @default.
• W28228725 cites W49737615 @default.
• W28228725 doi "https://doi.org/10.1007/978-1-61779-452-0_14" @default.
• W28228725 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22144309" @default.
• W28228725 hasPublicationYear "2011" @default.
• W28228725 type Work @default.
• W28228725 sameAs 28228725 @default.
• W28228725 citedByCount "23" @default.
• W28228725 countsByYear W282287252012 @default.

• next