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- W2824003057 abstract "The human gut microbiota is a crucial factor for the host’s physiology with respect to health and disease. Metagenomic shotgun sequencing of microbial gut communities revealed that Prevotella copri is one of the most important players in the gastrointestinal tract of many individuals. Because of the importance of this bacterium we analyzed the growth behavior and the central metabolic pathways of P. copri. Bioinformatic data, transcriptome profiling and enzyme activity measurements indicated that the major pathways are based on glycolysis and succinate production from fumarate. In addition, pyruvate can be degraded to acetate and formate. Electron transport phosphorylation depends on fumarate respiration with NADH and reduced ferredoxin as electron donors. In contrast to Bacteroides vulgatus, P. copri showed a more pronounced dependency on the addition of CO2 or bicarbonate for biomass formation, which is a remarkable difference between P. copri and Bacteroides spp. with important implication in the context of gut microbial competition. The analysis of substrate consumption and product concentrations from many P. copri cultures with different optical densities allowed a prediction of the carbon and electron flow in the central metabolism and a detailed calculation of growth yields as well as carbon and redox balances." @default.
- W2824003057 created "2018-07-19" @default.
- W2824003057 creator A5007137831 @default.
- W2824003057 creator A5076960142 @default.
- W2824003057 date "2018-08-01" @default.
- W2824003057 modified "2023-10-05" @default.
- W2824003057 title "Physiology and central carbon metabolism of the gut bacterium<i>Prevotella copri</i>" @default.
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- W2824003057 doi "https://doi.org/10.1111/mmi.14058" @default.
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