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- W2827886 abstract "Publisher Summary The molecular biology of membranes is an important and active area of science. The principal components of membranes and of the cell surfaces of many organisms are proteins and lipid-like molecules. This chapter presents a number of physical techniques that have been used to investigate the structural and dynamic properties of lipids, proteins, and membranes. Some of these techniques are X-ray diffraction, neutron diffraction, electron microscopy, differential scanning calorimetry, Raman spectroscopy, optical rotary dispersion, fluorescence spectroscopy, nuclear magnetic resonance (NMR), and electron paramagnetic resonance employing nitroxide spin labels. NMR spectroscopy has been applied mainly to the polar membrane phospholipids. Such polar lipids usually exist in bilayers whether dispersed individually in water or present in biological membranes. Lipid bilayers undergo phase transitions with changing temperature. At higher temperatures, they exist in a fluid-like liquid crystalline state while at lower temperatures, they are characterized by a gel-like state. The first NMR studies of lipids used the most abundant nucleus of lipids, hydrogen-1. The broad lines in the 1 H NMR spectra of lipids consist of many overlapping proton pair contributions, with each resonance broadened by interpair interactions. One way to resolve the intrapair and interpair interactions is to orient the lipids between glass slides and record the spectrum as a function of the orientation with respect to the magnetic field. It was found that the lipid chains can be divided roughly into two parts: (1) a less ordered part in the interior of the bilayer and (2) a more ordered part in the upper half of the chain" @default.
- W2827886 created "2016-06-24" @default.
- W2827886 creator A5009400572 @default.
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- W2827886 date "1985-01-01" @default.
- W2827886 modified "2023-09-23" @default.
- W2827886 title "19F NMR Investigations of Membranes" @default.
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