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- W2828538099 abstract "ABSTRACT A typical molecular cloning procedure requires Sanger sequencing for validation, which becomes cost-prohibitive and labour-intensive for large-scale clonal analysis of genotype-phenotype studies. Here we present a Tn5-mediated clonal analysis platform TnClone, which uses next-generation sequencing (NGS) to rapidly and cost-effectively analyze a large number of clones. We also developed a user-friendly graphical user interface and have provided general guidelines for conducting validation experiments. Using TnClone, we achieved more than 20-fold cost reduction compared with the cost incurred using conventional Sanger sequencing and detected low-frequency mutant clones (~10%) in mixed samples. We tested our programme and achieved 99.4% sensitivity. Our platform provides rapid turnaround with minimal hands-on time for secondary evaluation as NGS technology continues to evolve." @default.
- W2828538099 created "2018-07-19" @default.
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- W2828538099 date "2018-07-11" @default.
- W2828538099 modified "2023-09-25" @default.
- W2828538099 title "TnClone: high-throughput clonal analysis using Tn5-mediated library construction and de novo assembly" @default.
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- W2828538099 doi "https://doi.org/10.1101/367045" @default.
- W2828538099 hasPublicationYear "2018" @default.
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