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- W2830527296 abstract "Thymic epithelial cells are one of the main components of the thymic microenvironment required for T-cell development. In the present work, we describe an efficient method free of enzymatic and facs-sorted methods to culture human medullary thymic epithelial cells without affecting the cell phenotypic, physiologic and functional features. Human mTECs are obtained by culturing thymic biopsies explants. After 7 days of primo-culture, mTEC keep their ability to express key molecules involved in immune tolerance processes such as autoimmune regulator, tissue specific antigens, chemokines and cytokines. In addition, the cells sensor their cultured environment and consequently adjust their gene expression network. Therefore, we describe and provide a human mTEC model that may be used to test the effect of various molecules on TEC homeostasis and physiology. This method should allow the investigations of the specificities and the knowledge of human mTECs in normal or pathological conditions and therefore discontinue the extrapolations done on the murine models." @default.
- W2830527296 created "2018-07-19" @default.
- W2830527296 creator A5003709937 @default.
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- W2830527296 creator A5071599068 @default.
- W2830527296 creator A5078239122 @default.
- W2830527296 creator A5080838663 @default.
- W2830527296 date "2018-07-23" @default.
- W2830527296 modified "2023-10-18" @default.
- W2830527296 title "Cultured Human Thymic-Derived Cells Display Medullary Thymic Epithelial Cell Phenotype and Functionality" @default.
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- W2830527296 doi "https://doi.org/10.3389/fimmu.2018.01663" @default.
- W2830527296 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6064927" @default.
- W2830527296 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30083154" @default.