Matches in SemOpenAlex for { <https://semopenalex.org/work/W283564993> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W283564993 endingPage "290" @default.
- W283564993 startingPage "279" @default.
- W283564993 abstract "The measurement of fluorescently labeled actin filament movement driven by mechanoenzymes (e.g. myosin) is an important methodology for the study of molecular motors. It is assumed that the filament velocity, Vf, is analogous to the unloaded shortening velocity, Vu, seen in muscle fibers. To evaluate this assumption we compared Vf to literature values for Vu with regard to the effects of [ATP], [ADP], [Pi], pH, ionic strength (10–150 mM) and temperature (15–30°C). Vf and Vu are quantitatively similar with respect to the effects of substrate and product concentrations and temperatures > 20°C. However, Vf is more sensitive to decreases in pH and temperatures < 20°C than is Vu. At ionic strengths of 50–150 mM, Vf and Vu exhibit similar ionic strength dependencies (decreasing with ionic strength). At ionic strengths < 50 mM, Vf is markedly reduced. Thus while Vf is a good analogue for Vu under certain conditions (elevated ionic strength and temperatures > 20°C), under others it is not. The results of motility assays must be cautiously interpreted.KeywordsIonic StrengthThin FilamentMotility AssayFilament VelocityRabbit PsoasThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves." @default.
- W283564993 created "2016-06-24" @default.
- W283564993 creator A5039158310 @default.
- W283564993 creator A5043012356 @default.
- W283564993 creator A5086529957 @default.
- W283564993 date "1993-01-01" @default.
- W283564993 modified "2023-10-17" @default.
- W283564993 title "Factors Affecting Filament Velocity in In Vitro Motility Assays and their Relation to Unloaded Shortening Velocity in Muscle Fibers" @default.
- W283564993 cites W1526403580 @default.
- W283564993 cites W1963976991 @default.
- W283564993 cites W1967496500 @default.
- W283564993 cites W1971274190 @default.
- W283564993 cites W1972357724 @default.
- W283564993 cites W1988798258 @default.
- W283564993 cites W1991208956 @default.
- W283564993 cites W1997224677 @default.
- W283564993 cites W2009649815 @default.
- W283564993 cites W2026836008 @default.
- W283564993 cites W2028207310 @default.
- W283564993 cites W2030547704 @default.
- W283564993 cites W2031940637 @default.
- W283564993 cites W2036271044 @default.
- W283564993 cites W2045024410 @default.
- W283564993 cites W2061919351 @default.
- W283564993 cites W2075057480 @default.
- W283564993 cites W2085261165 @default.
- W283564993 cites W2140421284 @default.
- W283564993 cites W2144872573 @default.
- W283564993 cites W2150924142 @default.
- W283564993 cites W89094098 @default.
- W283564993 doi "https://doi.org/10.1007/978-1-4615-2872-2_27" @default.
- W283564993 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8109342" @default.
- W283564993 hasPublicationYear "1993" @default.
- W283564993 type Work @default.
- W283564993 sameAs 283564993 @default.
- W283564993 citedByCount "18" @default.
- W283564993 countsByYear W2835649932012 @default.
- W283564993 countsByYear W2835649932013 @default.
- W283564993 countsByYear W2835649932015 @default.
- W283564993 countsByYear W2835649932018 @default.
- W283564993 countsByYear W2835649932019 @default.
- W283564993 crossrefType "book-chapter" @default.
- W283564993 hasAuthorship W283564993A5039158310 @default.
- W283564993 hasAuthorship W283564993A5043012356 @default.
- W283564993 hasAuthorship W283564993A5086529957 @default.
- W283564993 hasConcept C113196181 @default.
- W283564993 hasConcept C12554922 @default.
- W283564993 hasConcept C125705527 @default.
- W283564993 hasConcept C14228908 @default.
- W283564993 hasConcept C145148216 @default.
- W283564993 hasConcept C178790620 @default.
- W283564993 hasConcept C184651966 @default.
- W283564993 hasConcept C185592680 @default.
- W283564993 hasConcept C187428577 @default.
- W283564993 hasConcept C192562407 @default.
- W283564993 hasConcept C2182769 @default.
- W283564993 hasConcept C43617362 @default.
- W283564993 hasConcept C55493867 @default.
- W283564993 hasConcept C6997183 @default.
- W283564993 hasConcept C86803240 @default.
- W283564993 hasConceptScore W283564993C113196181 @default.
- W283564993 hasConceptScore W283564993C12554922 @default.
- W283564993 hasConceptScore W283564993C125705527 @default.
- W283564993 hasConceptScore W283564993C14228908 @default.
- W283564993 hasConceptScore W283564993C145148216 @default.
- W283564993 hasConceptScore W283564993C178790620 @default.
- W283564993 hasConceptScore W283564993C184651966 @default.
- W283564993 hasConceptScore W283564993C185592680 @default.
- W283564993 hasConceptScore W283564993C187428577 @default.
- W283564993 hasConceptScore W283564993C192562407 @default.
- W283564993 hasConceptScore W283564993C2182769 @default.
- W283564993 hasConceptScore W283564993C43617362 @default.
- W283564993 hasConceptScore W283564993C55493867 @default.
- W283564993 hasConceptScore W283564993C6997183 @default.
- W283564993 hasConceptScore W283564993C86803240 @default.
- W283564993 hasLocation W2835649931 @default.
- W283564993 hasLocation W2835649932 @default.
- W283564993 hasOpenAccess W283564993 @default.
- W283564993 hasPrimaryLocation W2835649931 @default.
- W283564993 hasRelatedWork W1764011482 @default.
- W283564993 hasRelatedWork W1926160321 @default.
- W283564993 hasRelatedWork W2032282000 @default.
- W283564993 hasRelatedWork W2045194248 @default.
- W283564993 hasRelatedWork W2076297915 @default.
- W283564993 hasRelatedWork W2087573052 @default.
- W283564993 hasRelatedWork W2092360065 @default.
- W283564993 hasRelatedWork W2165627420 @default.
- W283564993 hasRelatedWork W2738108552 @default.
- W283564993 hasRelatedWork W2971551249 @default.
- W283564993 isParatext "false" @default.
- W283564993 isRetracted "false" @default.
- W283564993 magId "283564993" @default.
- W283564993 workType "book-chapter" @default.