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- W283699100 abstract "The microsome fraction isolated from rat liver homogenates by differential centrifugation was subfractionated by centrifuging in four different density gradients under conditions sufficient to bring the particles close to their equilibrium positions. The gradients were made up by means of a linearly increasing concentration of sucrose with either H2O or D2O as solvent, or of Ficoll with either 0.25 or 0.5 M sucrose as solvent. Some 15 subfractions were separated and analyzed for a number of enzymes and chemical constituents. The distribution patterns observed in these experiments allow the assayed components to be classified into four distinct groups of increasing median density: Monoamine oxidase, 5′-nucleotidase (assayed on AMP), and cholesterol. The two enzymes in this group behaved somewhat differently in different gradients and could be associated with distinct components. NADH-cytochrome c reductase, NADPH-cytochrome c reductase, aminopyrine demethylase, and phospholipids. Glucose-6-phosphatase, esterase, nucleoside diphosphatase (assayed on IDP, UDP, or GDP), and proteins. Fumarase, aldolase, glutamine synthetase, and RNA. The enzymes in group (d) all occur in significant amounts in the soluble fraction; their partial attachment to the RNA of ribosomes may be due to an adsorption artifact. On the other hand, the enzymes of group (a) are sufficiently dissociated from the others to suggest association with distinct membrane components present in small amounts in the preparation. Those bearing 5′-nucleotidase could be related to the plasma membrane, those containing monoamine oxidase to the outer mitrochondrial membrane. From the shape of their distributions, the enzymes of groups (b) and (c) all appear to belong largely or entirely to the main component of the preparation, i.e. to endoplasmic reticulum vesicles of both the smooth and the rough variety. The slight but significant differences in distribution between the two groups could reflect the existence either of a moderate degree of enzymic heterogeneity within a single membrane component, or of two distinct components each of which could be biochemically homogeneous." @default.
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- W283699100 date "1969-01-01" @default.
- W283699100 modified "2023-10-16" @default.
- W283699100 title "SUBFRACTIONATION OF RAT LIVER MICROSOMES" @default.
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- W283699100 doi "https://doi.org/10.1016/b978-1-4832-3223-2.50007-0" @default.
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