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• W284295240 abstract "Fusarium head blight (FHB), has been the most destructive disease of wheat, and producing the mycotoxin deoxynivalenol (DON), a protein synthesis inhibitor, which is harmful to humans and livestock. Previous studies have indicated that over-expression of yeast acetyl transferase gene (ScAYT1) encoding a 3-OH trichothecene acetyl transferase that converts DON to a less toxic acetylated form, leads to suppression of the DON sensitivity. In this study, we tested whether over-expression in tobacco transgenic plants can also increase toxin resistance. Thus, we introduced AYT1 into the model tobacco plants through Agrobacterium-mediated transformation in an attempt to detoxify DON. In vitro tests with extraction of normal and dsRNA carrying isolates of F. graminearum and 10 ppm of DON indicated variable resistance levels in transgenic plants and formation of 3AcDON upon DON treatment was confirmed by TLC method." @default.
• W284295240 created "2016-06-24" @default.
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• W284295240 date "2009-01-01" @default.
• W284295240 modified "2023-09-24" @default.
• W284295240 title "Transformed tobacco expressing ScAYT1 detoxifies deoxynivalenol in extract of dsRNA and cured isolates of Fusarium graminearum." @default.
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