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• W28532047 abstract "Concentrations and staining methods for identification of Giardia parasites in faecalmaterials from humans and animals are still the routine methods of diagnosis ofgiardiasis. Introduction of new and sensitive immunological and molecular methodswill definitely facilitate diagnosis and the identification of various Giardia speciesthat will ultimately improve clinical management and control of diseasetransmission. The identification of specific proteins of Giardia parasites, which aregenus and species specific, may further improve the specificity and sensitivity ofdiagnostic methods. In this study, identification and confirmation of the species ofGiardia parasite found in Malaysia, particularly in humans, dogs and rodents weredone by Polymerase Chain Reaction (PCR) using species-specific primers. SpecificHeat-Shock Protein (HSP) as markers for species identification were done usingSodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) andWestern Immunoblotting (WI). These markers were used as antigens for the production of species-specific monoclonal antibodies (MAb). The objectives of thisstudy were: i) to identify the common Giardia sp. infecting humans and othermammals (dogs and rodents) in Malaysia using specific Giardia primers by PCR; ii)to produce in vitro and detect immunogenic HSP using polyclonal sera from rabbitimmunised with antigens from known Giardia species on WI, and iii) to producespecies-specific MAb from the identified HSP markers. Microscopically, this studyhas observed that G. intestinalis (GI) and G. duodenalis (GD) were indistinguishablebut G. muris (GM) can be distinguished from GI or GD. In addition, this study hasalso confirmed that PCR using species-specific primers was more reliable andaccurate in detecting the variant of GI found in humans and dogs. GD isolatesrecovered from dogs was found to be the actual variant of GI of humans. Clearmorphological differentiations and identifications of GM and GI based onmicroscopical examination were observed and similar results were obtained by PCRusing species-specific primers of respective species of Giardia. However, the SDSPAGEand WI failed to identify species-specific HSP markers, but WI usingimmunised rabbit sera detected four immunogenic HSP, with the molecular weightof 30 kDa, 34 kDa, 58 kDa and 66 kDa. These four immunogenic HSP weredetected at 25°C, 37 °c and 50°C in both GI and GM. Three species-specificMAbs were produced using the combinations of the four immunogenic HSPs asantigens. These MAbs were designated as (i) [32 kDa HSPMAbGi(IgG3)], (ii) [29kDa HSPMAbGm(IgM)], and (iii) [20 kDa HSPMAbGi(IgGl)]. [32 kDaHSPMAbGi(IgG3)] MAb was specific for GI variant found in humans, [29 kDaHSPMAbGm(IgM)] MAb was specific for GM and [20 kDa HSPMAbGi(IgGl)]was specific for GI variant in both humans and dogs. These findings suggest that GIis the main causative agent of giardiasis in both humans and urban dogs in Malaysia GM is the main Giardia parasite infecting rodents in both rural and urban areas inMalaysia." @default.
• W28532047 created "2016-06-24" @default.
• W28532047 creator A5079615612 @default.
• W28532047 date "2004-10-01" @default.
• W28532047 modified "2023-09-26" @default.
• W28532047 title "Molecular and Immunological Identifications of Giardia Sp. Isolated From Humans, Dogs and Rodents" @default.
• W28532047 hasPublicationYear "2004" @default.
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