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- W2855354078 abstract "Ultrasonic disintegration is not among the most studied methodologies used for the purpose of cell disruption, according to the literature. The effect of Aspergillus flavus mycelium pre-treatment on ultrasonic disintegration efficiency, as well as optimisation of ultrasonic disintegration conditions for complete release of intracellular components without protein denaturation and loss of enzyme activity, was investigated. The results of ultrasonic disintegration monitored by microscopic analysis, protein quantification and determination of catalase activity were used to define a set of optimal working conditions: pre-treatment application of both quartz sand and lysozyme degradation of cell wall followed by ultrasonic processing for 5 minutes at full cycle and maximal amplitude. Under such conditions of ultrasonic homogenisation, extract obtained after 5 minute homogenization and extract of sediment additionally homogenized for 2 minutes contained a total of 87 % proteins, and 88 % enzyme activity." @default.
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- W2855354078 date "2010-01-01" @default.
- W2855354078 modified "2023-09-27" @default.
- W2855354078 title "Influence of Aspergillus flavus mycelium pre-treatment on ultrasonic disintergration efficiency" @default.
- W2855354078 hasPublicationYear "2010" @default.
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