Matches in SemOpenAlex for { <https://semopenalex.org/work/W286010845> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W286010845 abstract "Fluorescence Recovery after Photobleaching (FRAP) is a versatile technique to determine diffusion coefficients of suitably labelled species in fields like pharmaceutical research, biophysics or macromolecular chemistry [1]. Basically, a FRAP-experiment is realized by means of bleaching a certain area of the sample by a short and intense laser irradiation. Afterwards, the temporal recovery of fluorescence intensity in the bleached region due to the diffusion of unbleached molecules from the surroundings into this region is monitored by excitation with a highly attenuated beam. The diffusion coefficient can be deduced from the rate of recovery after suitable calibration. This procedure forms the basis for a variety of classical approaches to the evaluation of FRAP-experiments [1]. However, when FRAP-experiments are performed on a confocal laser scanning microscope (CLSM), the recovery process can be followed with high spatial resolution besides temporal resolution. Utilizing this aspect for a systematic analysis of FRAP data enables us to estimate the diffusion coefficient and the dimensionality of the diffusion process without the need for calibration [2]. This presentation shows the fundamentals of the latter approach. One- and two-dimensional diffusion processes (starting from a plane or a line source of the diffusing bleached species, respectively) can be readily realized on a CLSM equipped with an objetive lens with low numerical aperture and accordant bleaching of a line- or a spot-pattern into the confocal plane. The fact that in reality the bleaching pulse is neither infinitely sharp (Dirac-shaped) nor infinitely short is taken into account by a proper shift of the experimental time scale. A number of experiments performed by using this method shows that the diffusion coefficient and the expected dimensionality are obtained with high accuracy and consistency. Furthermore, using the temporal and spatial FRAP-information enables us to estimate multiple diffusion coefficients in experiments where more than one diffusing species is involved. This approach is extended to deduce the distribution of diffusion coefficients from one multi-component FRAP-experiment without any need for previous calibration." @default.
- W286010845 created "2016-06-24" @default.
- W286010845 creator A5011656288 @default.
- W286010845 creator A5037684295 @default.
- W286010845 date "2005-01-01" @default.
- W286010845 modified "2023-09-27" @default.
- W286010845 title "SYSTEMATIC EVALUATION OF FRAP-EXPERIMENTS AS A FUNCTION OF TIME AND SPACE PERFORMED ON A CONFOCAL LASER SCANNING MICROSCOPE" @default.
- W286010845 cites W2160675920 @default.
- W286010845 cites W278377992 @default.
- W286010845 hasPublicationYear "2005" @default.
- W286010845 type Work @default.
- W286010845 sameAs 286010845 @default.
- W286010845 citedByCount "0" @default.
- W286010845 crossrefType "journal-article" @default.
- W286010845 hasAuthorship W286010845A5011656288 @default.
- W286010845 hasAuthorship W286010845A5037684295 @default.
- W286010845 hasConcept C113196181 @default.
- W286010845 hasConcept C120665830 @default.
- W286010845 hasConcept C121332964 @default.
- W286010845 hasConcept C136009344 @default.
- W286010845 hasConcept C138268822 @default.
- W286010845 hasConcept C154945302 @default.
- W286010845 hasConcept C165838908 @default.
- W286010845 hasConcept C185592680 @default.
- W286010845 hasConcept C192562407 @default.
- W286010845 hasConcept C26659971 @default.
- W286010845 hasConcept C31332276 @default.
- W286010845 hasConcept C41008148 @default.
- W286010845 hasConcept C43617362 @default.
- W286010845 hasConcept C520434653 @default.
- W286010845 hasConcept C62520636 @default.
- W286010845 hasConcept C67649825 @default.
- W286010845 hasConcept C69357855 @default.
- W286010845 hasConcept C91881484 @default.
- W286010845 hasConcept C97355855 @default.
- W286010845 hasConceptScore W286010845C113196181 @default.
- W286010845 hasConceptScore W286010845C120665830 @default.
- W286010845 hasConceptScore W286010845C121332964 @default.
- W286010845 hasConceptScore W286010845C136009344 @default.
- W286010845 hasConceptScore W286010845C138268822 @default.
- W286010845 hasConceptScore W286010845C154945302 @default.
- W286010845 hasConceptScore W286010845C165838908 @default.
- W286010845 hasConceptScore W286010845C185592680 @default.
- W286010845 hasConceptScore W286010845C192562407 @default.
- W286010845 hasConceptScore W286010845C26659971 @default.
- W286010845 hasConceptScore W286010845C31332276 @default.
- W286010845 hasConceptScore W286010845C41008148 @default.
- W286010845 hasConceptScore W286010845C43617362 @default.
- W286010845 hasConceptScore W286010845C520434653 @default.
- W286010845 hasConceptScore W286010845C62520636 @default.
- W286010845 hasConceptScore W286010845C67649825 @default.
- W286010845 hasConceptScore W286010845C69357855 @default.
- W286010845 hasConceptScore W286010845C91881484 @default.
- W286010845 hasConceptScore W286010845C97355855 @default.
- W286010845 hasLocation W2860108451 @default.
- W286010845 hasOpenAccess W286010845 @default.
- W286010845 hasPrimaryLocation W2860108451 @default.
- W286010845 hasRelatedWork W1522235739 @default.
- W286010845 hasRelatedWork W1966599309 @default.
- W286010845 hasRelatedWork W1990639801 @default.
- W286010845 hasRelatedWork W2008674896 @default.
- W286010845 hasRelatedWork W2032894112 @default.
- W286010845 hasRelatedWork W2043179245 @default.
- W286010845 hasRelatedWork W2047697464 @default.
- W286010845 hasRelatedWork W2050519464 @default.
- W286010845 hasRelatedWork W2091068543 @default.
- W286010845 hasRelatedWork W2094806856 @default.
- W286010845 hasRelatedWork W2098017614 @default.
- W286010845 hasRelatedWork W2098384643 @default.
- W286010845 hasRelatedWork W2112892503 @default.
- W286010845 hasRelatedWork W2128075455 @default.
- W286010845 hasRelatedWork W2142247511 @default.
- W286010845 hasRelatedWork W2161173757 @default.
- W286010845 hasRelatedWork W2361072120 @default.
- W286010845 hasRelatedWork W2499476303 @default.
- W286010845 hasRelatedWork W2531923721 @default.
- W286010845 hasRelatedWork W3028162193 @default.
- W286010845 isParatext "false" @default.
- W286010845 isRetracted "false" @default.
- W286010845 magId "286010845" @default.
- W286010845 workType "article" @default.