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- W286091108 abstract "The sugarcane transformation programme at SASEX depends on the use of a negative selection gene, which confers resistance to the antibiotic geneticin. Expression of this antibiotic resistance gene results in the selective development of transgenic cells on a medium containing the antibiotic, which is lethal to the untransformed cells (negative selection). The presence of the resistance gene is superfluous and undesirable once the transgenic plant has been produced, and its elimination is a priority for the future release of commercially acceptable genetically modified sugarcane plants. Alternatively, a positive selection gene such as the manA gene that encodes the enzyme phosphomannose isomerase (PMI) can be employed. When expressed, the manA gene confers a metabolic advantage on the transgenic cells, enabling them to grow on a mannose-containing medium. Initial results indicated that a mixture of mannose and sucrose is needed to support callus growth. MS medium supplemented with 5 g/L mannose and 10 g/L sucrose was shown to prevent growth of untransformed sugarcane cells and was subsequently tested for the selection of sugarcane calli transformed with the manA gene. Putative transformants have been regenerated from the mannose selection regime tested, namely 5 g/L mannose in combination with either 5 or 10 g/L sucrose." @default.
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- W286091108 date "2004-01-01" @default.
- W286091108 modified "2023-09-26" @default.
- W286091108 title "Towards antibiotic resistance-free transgenic sugarcane." @default.
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