FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2864094541> ?p ?o ?g. }

• W2864094541 abstract "One approach to cell differentiation is to use the natural capacity of pluripotent stem cells to form three germ layers via embryoid bodies (EB). However, unification of this process during in vitro culture remains challenging and many microenvironmental factors including the number of cells in the culture can influence differentiation patterns. The number of cells serves a crucial role as it determines access to nutrients, the distribution of oxygen concentration and cellular interactions, all of which influence the fate of the differentiated cells. The influence of EBs derived from human pluripotent cells on the chondrogenic potential of such cells is not well understood. For this reason, the present study sought to determine the effect of varying amounts of cells on the properties of EBs derived from human embryonic stem cells (BG01V cell line). In the present study, 500‑2,000 cells per well were cultivated from 5 to 15 days in suspension cell culture. Expression of pluripotency genes and germ layer markers were evaluated in order to determine the EBs with the greatest and least mesodermal properties. Genes associated with pluripotency and chondrogenesis were also evaluated to assess the influence of suspension culture duration and EB size on chondrogenic differentiation. Immunofluorescence staining for pluripotent and chondrocyte‑associated proteins confirmed successful differentiation into chondrocyte‑like cells. Alcian blue staining confirmed deposition of proteoglycans. These results suggested that EBs formed in 500‑cell wells possess the highest mesodermal and prochondrogenic properties. Differentiation of EBs into chondrocytes on day 5 in 500‑cell wells was more efficient than in that observed in larger and older EBs." @default.
• W2864094541 created "2018-07-19" @default.
• W2864094541 creator A5016272718 @default.
• W2864094541 creator A5020265542 @default.
• W2864094541 creator A5032793898 @default.
• W2864094541 creator A5041866274 @default.
• W2864094541 creator A5044061571 @default.
• W2864094541 creator A5045322538 @default.
• W2864094541 date "2018-07-10" @default.
• W2864094541 modified "2023-09-30" @default.
• W2864094541 title "Effect of cellular mass on chondrogenic differentiation during embryoid body formation" @default.
• W2864094541 cites W101350248 @default.
• W2864094541 cites W1972075005 @default.
• W2864094541 cites W1983584466 @default.
• W2864094541 cites W2000335115 @default.
• W2864094541 cites W2002791185 @default.
• W2864094541 cites W2010353459 @default.
• W2864094541 cites W2013498422 @default.
• W2864094541 cites W2015052584 @default.
• W2864094541 cites W2025764784 @default.
• W2864094541 cites W2025972699 @default.
• W2864094541 cites W2030412907 @default.
• W2864094541 cites W2039829008 @default.
• W2864094541 cites W2060604173 @default.
• W2864094541 cites W2061656311 @default.
• W2864094541 cites W2072811404 @default.
• W2864094541 cites W2074783805 @default.
• W2864094541 cites W2084425498 @default.
• W2864094541 cites W2087178104 @default.
• W2864094541 cites W2088640544 @default.
• W2864094541 cites W2091298400 @default.
• W2864094541 cites W2107277218 @default.
• W2864094541 cites W2109460321 @default.
• W2864094541 cites W2114609816 @default.
• W2864094541 cites W2130073732 @default.
• W2864094541 cites W2133000252 @default.
• W2864094541 cites W2140395408 @default.
• W2864094541 cites W2145089654 @default.
• W2864094541 cites W2146224425 @default.
• W2864094541 cites W2158511728 @default.
• W2864094541 cites W2161275243 @default.
• W2864094541 cites W2169962294 @default.
• W2864094541 cites W2169980572 @default.
• W2864094541 cites W2173311271 @default.
• W2864094541 cites W2226963678 @default.
• W2864094541 cites W2563190195 @default.
• W2864094541 cites W1964184380 @default.
• W2864094541 doi "https://doi.org/10.3892/mmr.2018.9272" @default.
• W2864094541 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6102628" @default.
• W2864094541 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30015965" @default.
• W2864094541 hasPublicationYear "2018" @default.
• W2864094541 type Work @default.
• W2864094541 sameAs 2864094541 @default.
• W2864094541 citedByCount "3" @default.
• W2864094541 countsByYear W28640945412019 @default.
• W2864094541 countsByYear W28640945412020 @default.
• W2864094541 crossrefType "journal-article" @default.
• W2864094541 hasAuthorship W2864094541A5016272718 @default.
• W2864094541 hasAuthorship W2864094541A5020265542 @default.
• W2864094541 hasAuthorship W2864094541A5032793898 @default.
• W2864094541 hasAuthorship W2864094541A5041866274 @default.
• W2864094541 hasAuthorship W2864094541A5044061571 @default.
• W2864094541 hasAuthorship W2864094541A5045322538 @default.
• W2864094541 hasBestOaLocation W28640945411 @default.
• W2864094541 hasConcept C104317684 @default.
• W2864094541 hasConcept C105702510 @default.
• W2864094541 hasConcept C107459253 @default.
• W2864094541 hasConcept C143948831 @default.
• W2864094541 hasConcept C145103041 @default.
• W2864094541 hasConcept C148738053 @default.
• W2864094541 hasConcept C18155467 @default.
• W2864094541 hasConcept C185592680 @default.
• W2864094541 hasConcept C26805974 @default.
• W2864094541 hasConcept C2780550940 @default.
• W2864094541 hasConcept C2781403057 @default.
• W2864094541 hasConcept C28328180 @default.
• W2864094541 hasConcept C35496256 @default.
• W2864094541 hasConcept C55493867 @default.
• W2864094541 hasConcept C86803240 @default.
• W2864094541 hasConcept C95444343 @default.
• W2864094541 hasConceptScore W2864094541C104317684 @default.
• W2864094541 hasConceptScore W2864094541C105702510 @default.
• W2864094541 hasConceptScore W2864094541C107459253 @default.
• W2864094541 hasConceptScore W2864094541C143948831 @default.
• W2864094541 hasConceptScore W2864094541C145103041 @default.
• W2864094541 hasConceptScore W2864094541C148738053 @default.
• W2864094541 hasConceptScore W2864094541C18155467 @default.
• W2864094541 hasConceptScore W2864094541C185592680 @default.
• W2864094541 hasConceptScore W2864094541C26805974 @default.
• W2864094541 hasConceptScore W2864094541C2780550940 @default.
• W2864094541 hasConceptScore W2864094541C2781403057 @default.
• W2864094541 hasConceptScore W2864094541C28328180 @default.
• W2864094541 hasConceptScore W2864094541C35496256 @default.
• W2864094541 hasConceptScore W2864094541C55493867 @default.
• W2864094541 hasConceptScore W2864094541C86803240 @default.
• W2864094541 hasConceptScore W2864094541C95444343 @default.
• W2864094541 hasLocation W28640945411 @default.
• W2864094541 hasLocation W28640945412 @default.
• W2864094541 hasLocation W28640945413 @default.
• W2864094541 hasLocation W28640945414 @default.

• next