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- W287282073 abstract "Three overlapping genomic clones covering 29.0 kilobases of cotton DNA were found to encompass a cluster of two presumptive osmotin genes (OSMI and OSMII) and two osmotin pseudogenes (OSMIII and OSMIV). A segment of 16,007 basepairs of genomic DNA was sequenced from the overlapping genomic clones (GenBank Accessions AY303690 and AF304007). The two cotton osmotin genes were found to have open reading frames of 729 basepairs without any introns, and would encode presumptive osmotin preproteins of 242 amino acids. The open reading frames of the genes are identical in sequence to two corresponding cDNA clones (GenBank Accessions AF192271 and AY301283). The two cDNA inserts are almost full-length, since one lacks codons for the four N-terminal amino acids, and the other cDNA insert lacks the coding region for the 34 N-terminal amino acids. The cotton osmotin preproteins can be identified as PR5 proteins from their similarities to the deduced amino acid sequences of other plant osmotin PR5 preproteins. The preproteins would have N-terminal signal sequences of 24 amino acids, and the mature 24 kilodalton isoforms would likely be targeted for extracellular secretion. Prospective promoter elements, including two ethylene response elements, implicated as being positive regulatory elements in the expression of a number of PR-proteins, occur in the 5'-flanking regions. The mature osmotin proteins accumulate in cotton plants treated with the inducers ethephon and hydrogen peroxide. Thus, the two cotton osmotin genes encode osmotin proteins. The coding regions of the two genes have been expressed and isolated as fusion polypeptides in a bacterial expression system. Binary constructs containing the open reading frames of the two osmotin genes under the control of the 35S CaMV promoter have been generated for eventual production of transgenic Arabidopsis and cotton plants for potential constitutive expression of the osmotin proteins for increased resistance against fungal pathogens." @default.
- W287282073 created "2016-06-24" @default.
- W287282073 creator A5069301360 @default.
- W287282073 date "2003-12-01" @default.
- W287282073 modified "2023-09-27" @default.
- W287282073 title "Analysis of a Cotton Gene Cluster for the Antifungal Protein Osmotin" @default.
- W287282073 cites W105447860 @default.
- W287282073 cites W1145285480 @default.
- W287282073 cites W1226822808 @default.
- W287282073 cites W1482422555 @default.
- W287282073 cites W1497802870 @default.
- W287282073 cites W1508745089 @default.
- W287282073 cites W1512257839 @default.
- W287282073 cites W1525759724 @default.
- W287282073 cites W1532284082 @default.
- W287282073 cites W1552108102 @default.
- W287282073 cites W1600400967 @default.
- W287282073 cites W161683505 @default.
- W287282073 cites W189092266 @default.
- W287282073 cites W1959742154 @default.
- W287282073 cites W1966985251 @default.
- W287282073 cites W1967999707 @default.
- W287282073 cites W1968606500 @default.
- W287282073 cites W1970621669 @default.
- W287282073 cites W1971739164 @default.
- W287282073 cites W1972428047 @default.
- W287282073 cites W1973240543 @default.
- W287282073 cites W1974267207 @default.
- W287282073 cites W1980993948 @default.
- W287282073 cites W1982994771 @default.
- W287282073 cites W1985673423 @default.
- W287282073 cites W1985976585 @default.
- W287282073 cites W1986138577 @default.
- W287282073 cites W1989145266 @default.
- W287282073 cites W1989167510 @default.
- W287282073 cites W1990336506 @default.
- W287282073 cites W1991837864 @default.
- W287282073 cites W1991917593 @default.
- W287282073 cites W1993374451 @default.
- W287282073 cites W1995096879 @default.
- W287282073 cites W1995310701 @default.
- W287282073 cites W1995420892 @default.
- W287282073 cites W1996776761 @default.
- W287282073 cites W1998871484 @default.
- W287282073 cites W2002346423 @default.
- W287282073 cites W2003041228 @default.
- W287282073 cites W2003457510 @default.
- W287282073 cites W2003511339 @default.
- W287282073 cites W2004054644 @default.
- W287282073 cites W2005336502 @default.
- W287282073 cites W2005610674 @default.
- W287282073 cites W2006733565 @default.
- W287282073 cites W2007351562 @default.
- W287282073 cites W2008045895 @default.
- W287282073 cites W2008065738 @default.
- W287282073 cites W2014483325 @default.
- W287282073 cites W2014669590 @default.
- W287282073 cites W2015673321 @default.
- W287282073 cites W2015733224 @default.
- W287282073 cites W2019725492 @default.
- W287282073 cites W2026569528 @default.
- W287282073 cites W2031687796 @default.
- W287282073 cites W2038305047 @default.
- W287282073 cites W2039642480 @default.
- W287282073 cites W2039761898 @default.
- W287282073 cites W2041014178 @default.
- W287282073 cites W2041450888 @default.
- W287282073 cites W2045639680 @default.
- W287282073 cites W2047501022 @default.
- W287282073 cites W2047775172 @default.
- W287282073 cites W2049661143 @default.
- W287282073 cites W2051341726 @default.
- W287282073 cites W2054901867 @default.
- W287282073 cites W2060742513 @default.
- W287282073 cites W2061091892 @default.
- W287282073 cites W2071543405 @default.
- W287282073 cites W2074204387 @default.
- W287282073 cites W2074910739 @default.
- W287282073 cites W2075960038 @default.
- W287282073 cites W2078269952 @default.
- W287282073 cites W2079468421 @default.
- W287282073 cites W2081531079 @default.
- W287282073 cites W2082157101 @default.
- W287282073 cites W2084035056 @default.
- W287282073 cites W2085418697 @default.
- W287282073 cites W2087470698 @default.
- W287282073 cites W2089575234 @default.
- W287282073 cites W2090244038 @default.
- W287282073 cites W2092686336 @default.
- W287282073 cites W2094366292 @default.
- W287282073 cites W2099052138 @default.
- W287282073 cites W2100837269 @default.
- W287282073 cites W2101648814 @default.
- W287282073 cites W2102271723 @default.
- W287282073 cites W2107804109 @default.
- W287282073 cites W2108134611 @default.
- W287282073 cites W2115912546 @default.
- W287282073 cites W2120020813 @default.
- W287282073 cites W2120231778 @default.
- W287282073 cites W2123159024 @default.