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• W2874575179 abstract "Abstract We investigated the dynamic changes in chromatin and microtubules at the first cell cycle in goat somatic cell nuclear transfer (SCNT)‐derived and in vitro fertilization (IVF)‐derived embryos. Stage‐dependent and characteristic changes to chromatin and microtubules occurred in SCNT‐derived embryos at different times after activation. About half donor nuclei underwent premature chromosome condensation (PCC) at 1 h post activation, and furtherly reached telophase at 2 h after activation. However, we discovered that the separated chromosomes reaggregated, not keeping two independent nuclei; and formed one pronucleus at 2.5 h after activation. One pronucleus was found in all reconstructed oocytes except other no nucleus oocytes from 3 to 22 h after activation. Reconstructed oocytes reached the first mitotic metaphase at 23 h post activation, which was later than that of IVF‐derived embryos at 16 h after insemination. SCNT‐derived embryos showed significantly higher abnormalities in the first mitotic metaphase spindle, compared with IVF‐derived embryos. Abnormal spindles included multi polar and half spindles. SCNT‐derived embryos began to cleave at 24 h after activation, which was later than that of IVF‐derived embryos at 21 h after insemination. SCNT‐derived embryos showed delayed conversion from telophase to interphase than IVF‐derived embryos during cleavage. These might lead to poor development in SCNT‐derived embryos." @default.
• W2874575179 created "2018-07-19" @default.
• W2874575179 creator A5066058965 @default.
• W2874575179 creator A5075886667 @default.
• W2874575179 date "2018-07-31" @default.
• W2874575179 modified "2023-10-18" @default.
• W2874575179 title "Dynamic changes in chromatin and microtubules at the first cell cycle in SCNT or IVF goat embryos" @default.
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• W2874575179 doi "https://doi.org/10.1002/cbin.11031" @default.
• W2874575179 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29993158" @default.
• W2874575179 hasPublicationYear "2018" @default.
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