Matches in SemOpenAlex for { <https://semopenalex.org/work/W287496613> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W287496613 abstract "Inflammatory bowel disease (IBD) comprises diseases that are characterized by chronic or relapsing inflammation of the gastrointestinal tract. Primary sclerosing cholangitis (PSC) is an extraintestinal manifestation in IBD. Immunoreactivity against an autoantigen that is expressed both in the gastrointestinal tract and the biliary tract could be the link between these diseases. A possible source of such an antigen is goblet cells. Immunostainings of normal human tissues using IBD patient sera showed goblet cell immunoreactivity against goblet cells in all parts of the gastrointestinal tract. The most frequent immunostaining was found against goblet cells in the appendix against which 84% (42/50) of IBD patients compared to 8% (4/50) of healthy blood donors showed immunoreactivity. To identify the corresponding antigen we used three different approaches, investigation of immunoreactivity to different candidate proteins compared to IBD sera, immunoscreening of an appendiceal cDNA library, and immunoprecipitation of protein lysates from mucin producing cells followed by SDS-PAGE and 2D gel electrophoresis. These approaches led to the identification of several candidate autoantigens of which complement C3 is the most promising. A novel staining pattern with strong immunoreactivity to granules and the apical membrane of biliary epithelial cells was identified with 35% (12/34) of PSC sera compared to none of healthy controls (n=28). Screening of a cDNA library from normal human choledochus identified PDZ domain containing 1 (Pdzk1) and Glutathion S transferase theta 1 (GSTT1) as potential candidates. Pdzk1 is an interesting candidate which is expressed in the intestinal tract and bile ducts. GSTT1 antibodies were not specific for PSC and are thought to develop as an alloimmune response in patients with the GSTT1-null genotype. In conclusion, we have identified specific immunoreactivity to goblet cells and biliary epithelial cells using sera from patients with IBD and PSC respectively. We have also identified several potential autoantigens." @default.
- W287496613 created "2016-06-24" @default.
- W287496613 creator A5021512169 @default.
- W287496613 creator A5028168158 @default.
- W287496613 creator A5078801536 @default.
- W287496613 creator A5080721477 @default.
- W287496613 creator A5081357043 @default.
- W287496613 creator A5084300940 @default.
- W287496613 date "2008-01-01" @default.
- W287496613 modified "2023-09-24" @default.
- W287496613 title "Investigation of goblet cell autoantigens in patients with inflammatory bowel disease." @default.
- W287496613 hasPublicationYear "2008" @default.
- W287496613 type Work @default.
- W287496613 sameAs 287496613 @default.
- W287496613 citedByCount "0" @default.
- W287496613 crossrefType "journal-article" @default.
- W287496613 hasAuthorship W287496613A5021512169 @default.
- W287496613 hasAuthorship W287496613A5028168158 @default.
- W287496613 hasAuthorship W287496613A5078801536 @default.
- W287496613 hasAuthorship W287496613A5080721477 @default.
- W287496613 hasAuthorship W287496613A5081357043 @default.
- W287496613 hasAuthorship W287496613A5084300940 @default.
- W287496613 hasConcept C126322002 @default.
- W287496613 hasConcept C142724271 @default.
- W287496613 hasConcept C147483822 @default.
- W287496613 hasConcept C159654299 @default.
- W287496613 hasConcept C179264091 @default.
- W287496613 hasConcept C203014093 @default.
- W287496613 hasConcept C204232928 @default.
- W287496613 hasConcept C2775941134 @default.
- W287496613 hasConcept C2775982439 @default.
- W287496613 hasConcept C2778260677 @default.
- W287496613 hasConcept C2779134260 @default.
- W287496613 hasConcept C2780479503 @default.
- W287496613 hasConcept C2780512811 @default.
- W287496613 hasConcept C4224716 @default.
- W287496613 hasConcept C529295009 @default.
- W287496613 hasConcept C71924100 @default.
- W287496613 hasConcept C86803240 @default.
- W287496613 hasConceptScore W287496613C126322002 @default.
- W287496613 hasConceptScore W287496613C142724271 @default.
- W287496613 hasConceptScore W287496613C147483822 @default.
- W287496613 hasConceptScore W287496613C159654299 @default.
- W287496613 hasConceptScore W287496613C179264091 @default.
- W287496613 hasConceptScore W287496613C203014093 @default.
- W287496613 hasConceptScore W287496613C204232928 @default.
- W287496613 hasConceptScore W287496613C2775941134 @default.
- W287496613 hasConceptScore W287496613C2775982439 @default.
- W287496613 hasConceptScore W287496613C2778260677 @default.
- W287496613 hasConceptScore W287496613C2779134260 @default.
- W287496613 hasConceptScore W287496613C2780479503 @default.
- W287496613 hasConceptScore W287496613C2780512811 @default.
- W287496613 hasConceptScore W287496613C4224716 @default.
- W287496613 hasConceptScore W287496613C529295009 @default.
- W287496613 hasConceptScore W287496613C71924100 @default.
- W287496613 hasConceptScore W287496613C86803240 @default.
- W287496613 hasLocation W2874966131 @default.
- W287496613 hasOpenAccess W287496613 @default.
- W287496613 hasPrimaryLocation W2874966131 @default.
- W287496613 hasRelatedWork W1782151952 @default.
- W287496613 hasRelatedWork W1910841180 @default.
- W287496613 hasRelatedWork W1970478161 @default.
- W287496613 hasRelatedWork W1996171052 @default.
- W287496613 hasRelatedWork W1996562778 @default.
- W287496613 hasRelatedWork W2005096573 @default.
- W287496613 hasRelatedWork W2071966826 @default.
- W287496613 hasRelatedWork W2110343451 @default.
- W287496613 hasRelatedWork W2115573101 @default.
- W287496613 hasRelatedWork W2116148756 @default.
- W287496613 hasRelatedWork W2118695466 @default.
- W287496613 hasRelatedWork W2126545869 @default.
- W287496613 hasRelatedWork W2149495029 @default.
- W287496613 hasRelatedWork W2155208550 @default.
- W287496613 hasRelatedWork W2162485461 @default.
- W287496613 hasRelatedWork W2165098240 @default.
- W287496613 hasRelatedWork W2327675008 @default.
- W287496613 hasRelatedWork W2359538803 @default.
- W287496613 hasRelatedWork W3023392672 @default.
- W287496613 hasRelatedWork W2157206581 @default.
- W287496613 isParatext "false" @default.
- W287496613 isRetracted "false" @default.
- W287496613 magId "287496613" @default.
- W287496613 workType "article" @default.