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- W287724008 abstract "Nowadays, the dependence that our society has to non-biodegradable plastics derivedfrom petroleum has created the necessity to develop an economically viable alternative.Biopolymers seem to be an attractive alternative to common plastics. Among all them,Polyhydroxyalkanoates (PHA) represent one of the most studied and important source ofbiopolymers. These biopolymers are produced naturally by various microorganism that usedifferent nutrient sources to conduct the production. Many studies are focusing their efforts inthe utilization of alternative substrates to reduce the final cost of the whole process. Besides,the utilization of transgenic organisms (obtained through genetic engineering techniques) forbiopolymers production seems to be the path to follow for a higher PHA production and todiminish the cost of the whole biosynthesis.Bearing in mind all this information, in the present thesis, a work to study all thedifferent aspects that involve the obtaining of PHB through fermentation in Ralstonia eutrophawas carried out.Hence, the thesis pretends to evaluate experimentally the obtaining, isolation andcharacterization of PHB in Ralstonia eutropha using different configurations and systems. Allthese experimental stages were carried out in Terrassa and Barcelona (UPC). Besides, thisstudy includes a theoretical part where all the aspects referred to PHB are analyzed andsupports the experimental work carried out.During the experimental part, three different fermentations with Ralstonia eutrophawere conducted. The main aim of these fermentations was to see the effect of time andglucose dosage in a fermentative process considering an initial medium –according toliterature- as standard.In all these processes, an extraction method with chloroform was used to isolate thefinal biopolymer. Afterward, a characterization of the product obtained by means of FTIR, NMRand GPC analysis was carried out. All these results are presented in the corresponding section.Besides, in the thesis, an economic study is included in the appendix section. This studycomprises the economic cost that is required to develop the PHB process described in theexperimental part. Thus, the economic cost, the income and the budget of all the differentaspects of the process are considered." @default.
- W287724008 created "2016-06-24" @default.
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- W287724008 date "2012-09-01" @default.
- W287724008 modified "2023-09-27" @default.
- W287724008 title "Biopolyester (PHB) obtaining by fermentation of Ralstonia eutropha" @default.
- W287724008 hasPublicationYear "2012" @default.
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