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- W2879138720 abstract "The capability of microorganisms to alter metal speciation offers potential for the development of new strategies for immobilization of toxic metals in the environment. A metal-reducing microbe, Pelosinus lilae strain UFO1, was isolated under strictly anaerobic conditions from an Fe(III)-reducing enrichment established with uncontaminated soil from the Department of Energy Oak Ridge Field Research Center, Tennessee. P. lilae UFO1 is a rod-shaped, spore-forming, and Gram-variable anaerobe with a fermentative metabolism. It is capable of reducing the humic acid analog anthraquinone-2,6-disulfonate (AQDS) using a variety of fermentable substrates and H2. Reduction of Fe(III)-nitrilotriacetic acid occurred in the presence of lactate as carbon and electron donor. Ferrihydrite was not reduced in the absence of AQDS. Nearly complete reduction of 1, 3, and 5 ppm Cr(VI) occurred within 24 h in suspensions containing 108 cells mL-1 when provided with 10 mM lactate; when 1 mM AQDS was added, 3 and 5 ppm Cr(VI) were reduced to 0.1 ppm within 2 h. Strain UFO1 is a novel species within the bacterial genus Pelosinus, having 98.16% 16S rRNA gene sequence similarity with the most closely related described species, Pelosinus fermentans R7T. The G+C content of the genomic DNA was 38 mol%, and DNA-DNA hybridization of P. lilae UFO1 against P. fermentans R7T indicated an average 16.8% DNA-DNA similarity. The unique phylogenetic, physiologic, and metal-transforming characteristics of P. lilae UFO1 reveal it is a novel isolate of the described genus Pelosinus." @default.
- W2879138720 created "2018-07-19" @default.
- W2879138720 creator A5041436482 @default.
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- W2879138720 date "2018-08-17" @default.
- W2879138720 modified "2023-09-27" @default.
- W2879138720 title "Metal Transformation by a Novel Pelosinus Isolate From a Subsurface Environment" @default.
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- W2879138720 doi "https://doi.org/10.3389/fmicb.2018.01689" @default.
- W2879138720 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6107796" @default.
- W2879138720 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30174652" @default.
- W2879138720 hasPublicationYear "2018" @default.
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