Matches in SemOpenAlex for { <https://semopenalex.org/work/W2881025773> ?p ?o ?g. }
- W2881025773 abstract "Abstract Single-particle electron cryo-microscopy and computational image classification can be used to analyze structural variability in macromolecules and their assemblies. In some cases, a particle may contain different regions that each display a range of distinct conformations. We have developed strategies, implemented within the Frealign and cis TEM image processing packages, to focus classify on specific regions of a particle and detect potential covariance. The strategies are based on masking the region of interest using either a 2-D mask applied to reference projections and particle images, or a 3-D mask applied to the 3-D volume. We show that focused classification approaches can be used to study structural allostery, a concept that is likely to gain more importance as datasets grow in size, allowing the distinction of more structural states and smaller differences between states. Finally, we apply the approaches to an experimental dataset containing the HIV-1 Transactivation Response (TAR) element RNA fused into the large bacterial ribosomal subunit, to deconvolve structural mobility within localized regions of interest. Highlights Description of different image classification strategies in single-particle cryo-EM Quantitative evaluation of two classification methods using simulated data Application of the two classification methods to an experimental dataset" @default.
- W2881025773 created "2018-07-19" @default.
- W2881025773 creator A5008530670 @default.
- W2881025773 creator A5024079309 @default.
- W2881025773 creator A5049506879 @default.
- W2881025773 creator A5054867967 @default.
- W2881025773 creator A5075596275 @default.
- W2881025773 creator A5077915889 @default.
- W2881025773 date "2018-07-28" @default.
- W2881025773 modified "2023-10-16" @default.
- W2881025773 title "Analysis of Local Variability and Allostery in Macromolecular Assemblies using Cryo-EM and Focused Classification" @default.
- W2881025773 cites W1832001947 @default.
- W2881025773 cites W1876329184 @default.
- W2881025773 cites W1976067034 @default.
- W2881025773 cites W2001923477 @default.
- W2881025773 cites W2006158980 @default.
- W2881025773 cites W2011061704 @default.
- W2881025773 cites W2024858227 @default.
- W2881025773 cites W2025759721 @default.
- W2881025773 cites W2031880123 @default.
- W2881025773 cites W2046563831 @default.
- W2881025773 cites W2050329965 @default.
- W2881025773 cites W2068090915 @default.
- W2881025773 cites W2068654340 @default.
- W2881025773 cites W2072064506 @default.
- W2881025773 cites W2072834874 @default.
- W2881025773 cites W2076449818 @default.
- W2881025773 cites W2104234755 @default.
- W2881025773 cites W2104382086 @default.
- W2881025773 cites W2120396539 @default.
- W2881025773 cites W2124026197 @default.
- W2881025773 cites W2131324684 @default.
- W2881025773 cites W2132629607 @default.
- W2881025773 cites W2140360168 @default.
- W2881025773 cites W2143859034 @default.
- W2881025773 cites W2148941629 @default.
- W2881025773 cites W2151234959 @default.
- W2881025773 cites W2152575748 @default.
- W2881025773 cites W2155596249 @default.
- W2881025773 cites W2158181541 @default.
- W2881025773 cites W2161661741 @default.
- W2881025773 cites W2212589734 @default.
- W2881025773 cites W2258912434 @default.
- W2881025773 cites W2275651183 @default.
- W2881025773 cites W2346206575 @default.
- W2881025773 cites W2411563451 @default.
- W2881025773 cites W2418037503 @default.
- W2881025773 cites W2432427150 @default.
- W2881025773 cites W2517678370 @default.
- W2881025773 cites W2550637017 @default.
- W2881025773 cites W2560111401 @default.
- W2881025773 cites W2568171404 @default.
- W2881025773 cites W2593511418 @default.
- W2881025773 cites W2616877598 @default.
- W2881025773 cites W2728385286 @default.
- W2881025773 cites W2736403501 @default.
- W2881025773 cites W2753200340 @default.
- W2881025773 cites W2766732551 @default.
- W2881025773 cites W2775458966 @default.
- W2881025773 cites W2791354025 @default.
- W2881025773 cites W2791617818 @default.
- W2881025773 cites W2798457309 @default.
- W2881025773 cites W2799415291 @default.
- W2881025773 cites W2950967305 @default.
- W2881025773 cites W2953320394 @default.
- W2881025773 cites W4239090871 @default.
- W2881025773 doi "https://doi.org/10.1101/365940" @default.
- W2881025773 hasPublicationYear "2018" @default.
- W2881025773 type Work @default.
- W2881025773 sameAs 2881025773 @default.
- W2881025773 citedByCount "0" @default.
- W2881025773 crossrefType "posted-content" @default.
- W2881025773 hasAuthorship W2881025773A5008530670 @default.
- W2881025773 hasAuthorship W2881025773A5024079309 @default.
- W2881025773 hasAuthorship W2881025773A5049506879 @default.
- W2881025773 hasAuthorship W2881025773A5054867967 @default.
- W2881025773 hasAuthorship W2881025773A5075596275 @default.
- W2881025773 hasAuthorship W2881025773A5077915889 @default.
- W2881025773 hasBestOaLocation W28810257731 @default.
- W2881025773 hasConcept C11413529 @default.
- W2881025773 hasConcept C124101348 @default.
- W2881025773 hasConcept C142362112 @default.
- W2881025773 hasConcept C153180895 @default.
- W2881025773 hasConcept C153349607 @default.
- W2881025773 hasConcept C154945302 @default.
- W2881025773 hasConcept C159985019 @default.
- W2881025773 hasConcept C174576160 @default.
- W2881025773 hasConcept C186060115 @default.
- W2881025773 hasConcept C18903297 @default.
- W2881025773 hasConcept C192562407 @default.
- W2881025773 hasConcept C204323151 @default.
- W2881025773 hasConcept C20702342 @default.
- W2881025773 hasConcept C2777402240 @default.
- W2881025773 hasConcept C2778517922 @default.
- W2881025773 hasConcept C41008148 @default.
- W2881025773 hasConcept C55493867 @default.
- W2881025773 hasConcept C86803240 @default.
- W2881025773 hasConceptScore W2881025773C11413529 @default.
- W2881025773 hasConceptScore W2881025773C124101348 @default.
- W2881025773 hasConceptScore W2881025773C142362112 @default.