Matches in SemOpenAlex for { <https://semopenalex.org/work/W2883004290> ?p ?o ?g. }
- W2883004290 abstract "Abstract Pluripotent stem cells (PSCs) edited with genetic reporters are useful tools for differentiation analysis and for isolation of specific cell populations for study. Reporter integration into the genome is now commonly achieved by targeted DNA nuclease-enhanced homology directed repair (HDR). However, human PSCs are known to have a low frequency of gene knock-in (KI) by HDR, making reporter line generation an arduous process. Here, we report a methodology for scarless KI of large fluorescent reporter genes into PSCs by transient selection with puromycin or zeocin. With this method, we can perform targeted KI of a single reporter gene with up to 65% efficiency, as well as simultaneous KI of two reporter genes into different loci with up to 11% efficiency. Additionally, we demonstrate that this method also works in mouse PSCs." @default.
- W2883004290 created "2018-08-03" @default.
- W2883004290 creator A5035743592 @default.
- W2883004290 creator A5040841311 @default.
- W2883004290 creator A5056252981 @default.
- W2883004290 creator A5063328522 @default.
- W2883004290 creator A5082603219 @default.
- W2883004290 creator A5083040877 @default.
- W2883004290 date "2018-07-23" @default.
- W2883004290 modified "2023-09-27" @default.
- W2883004290 title "Highly efficient scarless knock-in of reporter genes into human and mouse pluripotent stem cells via transient antibiotic selection" @default.
- W2883004290 cites W1496067884 @default.
- W2883004290 cites W1528466007 @default.
- W2883004290 cites W1596255224 @default.
- W2883004290 cites W1958673105 @default.
- W2883004290 cites W1990149668 @default.
- W2883004290 cites W1992822725 @default.
- W2883004290 cites W2007594606 @default.
- W2883004290 cites W2050612189 @default.
- W2883004290 cites W2060837465 @default.
- W2883004290 cites W2070783056 @default.
- W2883004290 cites W2072127633 @default.
- W2883004290 cites W2112444085 @default.
- W2883004290 cites W2114232807 @default.
- W2883004290 cites W2116534141 @default.
- W2883004290 cites W2145782793 @default.
- W2883004290 cites W2149884610 @default.
- W2883004290 cites W2150273306 @default.
- W2883004290 cites W2160331473 @default.
- W2883004290 cites W2162573011 @default.
- W2883004290 cites W2162603028 @default.
- W2883004290 cites W2163248265 @default.
- W2883004290 cites W2165838325 @default.
- W2883004290 cites W2168487117 @default.
- W2883004290 cites W2267596434 @default.
- W2883004290 cites W2319627288 @default.
- W2883004290 cites W2338408645 @default.
- W2883004290 cites W2345282885 @default.
- W2883004290 cites W2409724652 @default.
- W2883004290 cites W2503342171 @default.
- W2883004290 cites W2515633032 @default.
- W2883004290 cites W2560890620 @default.
- W2883004290 cites W2565787913 @default.
- W2883004290 cites W2590222835 @default.
- W2883004290 cites W2590493137 @default.
- W2883004290 cites W2601350462 @default.
- W2883004290 cites W2604275021 @default.
- W2883004290 cites W2619894622 @default.
- W2883004290 cites W2761739377 @default.
- W2883004290 cites W2781815848 @default.
- W2883004290 cites W2791013442 @default.
- W2883004290 cites W2795836363 @default.
- W2883004290 cites W2796382741 @default.
- W2883004290 cites W2803616467 @default.
- W2883004290 doi "https://doi.org/10.1101/374728" @default.
- W2883004290 hasPublicationYear "2018" @default.
- W2883004290 type Work @default.
- W2883004290 sameAs 2883004290 @default.
- W2883004290 citedByCount "1" @default.
- W2883004290 countsByYear W28830042902021 @default.
- W2883004290 crossrefType "posted-content" @default.
- W2883004290 hasAuthorship W2883004290A5035743592 @default.
- W2883004290 hasAuthorship W2883004290A5040841311 @default.
- W2883004290 hasAuthorship W2883004290A5056252981 @default.
- W2883004290 hasAuthorship W2883004290A5063328522 @default.
- W2883004290 hasAuthorship W2883004290A5082603219 @default.
- W2883004290 hasAuthorship W2883004290A5083040877 @default.
- W2883004290 hasBestOaLocation W28830042901 @default.
- W2883004290 hasConcept C104317684 @default.
- W2883004290 hasConcept C107459253 @default.
- W2883004290 hasConcept C111829913 @default.
- W2883004290 hasConcept C145103041 @default.
- W2883004290 hasConcept C150194340 @default.
- W2883004290 hasConcept C153911025 @default.
- W2883004290 hasConcept C161733203 @default.
- W2883004290 hasConcept C2779200571 @default.
- W2883004290 hasConcept C2796436 @default.
- W2883004290 hasConcept C3675279 @default.
- W2883004290 hasConcept C54355233 @default.
- W2883004290 hasConcept C70721500 @default.
- W2883004290 hasConcept C77957584 @default.
- W2883004290 hasConcept C86803240 @default.
- W2883004290 hasConcept C95444343 @default.
- W2883004290 hasConceptScore W2883004290C104317684 @default.
- W2883004290 hasConceptScore W2883004290C107459253 @default.
- W2883004290 hasConceptScore W2883004290C111829913 @default.
- W2883004290 hasConceptScore W2883004290C145103041 @default.
- W2883004290 hasConceptScore W2883004290C150194340 @default.
- W2883004290 hasConceptScore W2883004290C153911025 @default.
- W2883004290 hasConceptScore W2883004290C161733203 @default.
- W2883004290 hasConceptScore W2883004290C2779200571 @default.
- W2883004290 hasConceptScore W2883004290C2796436 @default.
- W2883004290 hasConceptScore W2883004290C3675279 @default.
- W2883004290 hasConceptScore W2883004290C54355233 @default.
- W2883004290 hasConceptScore W2883004290C70721500 @default.
- W2883004290 hasConceptScore W2883004290C77957584 @default.
- W2883004290 hasConceptScore W2883004290C86803240 @default.
- W2883004290 hasConceptScore W2883004290C95444343 @default.
- W2883004290 hasLocation W28830042901 @default.
- W2883004290 hasLocation W28830042902 @default.