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• W2883056464 abstract "The sequence assembly process can be divided into three stages: contigs extension, scaffolding, and gap filling. The scaffolding method is an essential step during the process to infer the direction and sequence relationships between the contigs. However, scaffolding still faces the challenges of uneven sequencing depth, genome repetitive regions, and sequencing errors, which often leads to many false relationships between contigs. The performance of scaffolding can be improved by removing potential false conjunctions between contigs. In this study, a novel scaffolding algorithm which is on the basis of path extension Loose-Strict-Loose strategy and contig error correction, called iLSLS. iLSLS helps reduce the false relationships between contigs, and improve the accuracy of subsequent steps. iLSLS utilizes a scoring function, which estimates the correctness of candidate paths by the distribution of paired reads, and try to conduction the extension with the path which is scored the highest. What's more, iLSLS can precisely estimate the gap size. We conduct experiments on two real datasets, and the results show that LSLS strategy is efficient to increase the correctness of scaffolds, and iLSLS performs better than other scaffolding methods." @default.
• W2883056464 created "2018-08-03" @default.
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• W2883056464 date "2019-05-01" @default.
• W2883056464 modified "2023-10-16" @default.
• W2883056464 title "A Novel Scaffolding Algorithm Based on Contig Error Correction and Path Extension" @default.
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• W2883056464 doi "https://doi.org/10.1109/tcbb.2018.2858267" @default.
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