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- W2883132877 abstract "To investigate fibrin-fibrinogen interactions at 37d°C, affinity chromatography was performed on fibrinogen-sepharose as well as fibrin-sepharose. As thrombin cleaves fibrinopeptides A and B from fibrinogen producing des-A fibrin and des-AB fibrin respectively, experiments were performed with both types of fibrin. Thrombin-induced, urea-solubilized des-AB 125I-fibrin as well as batroxobin-induced d es-A 125I-fibrin were mixed with plasma containing 131I-fibrinogen. These mixtures were passed through fibrinogensepharose columns equilibrated with tris buffer,pH 7.4, containing EDTA, benzamidine, aprotinin and NaN3 . The elution buffer (buffer B) contained tris and guanidine, pH 4.1. Results: At 37°C, des-A fibrin passed through the column together with fibrinogen, whereas des-AB fibrin stuck to the immobilized fibrinogen. In the next set of experiments, fibrin-fibrinogen mixtures were chromatographed on des-AB fibrin-sepharose . R e sults: At 20°C as well as at 37°C, des-A fibrin as well as des-AB fibrin were eluted together with fibrinogen with buffer B. In the last set of experiments, fibrin-fibrinogen mixtures were chromatographed on des-A fibrin-sepharose columns. Results: At 37°C, des-A fibrin as well as des-AB fibrin interacted with immobilized des-A fibrin, whereas fibrinogen did not stick to des-A fibrin. The results suggest that des-AB fibrin interacts with fibrinogen at 37°C. An interaction, if any, between des-A fibrin and fibrinogen is so weak at 37°C that it cannot be detected by affinity chromatography." @default.
- W2883132877 created "2018-08-03" @default.
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- W2883132877 date "1981-01-01" @default.
- W2883132877 modified "2023-09-23" @default.
- W2883132877 title "Different Affinity Of Fibrinogen To Des-A Fibrin And Des-Ab Fibrin At 37°C" @default.
- W2883132877 doi "https://doi.org/10.1055/s-0038-1652439" @default.
- W2883132877 hasPublicationYear "1981" @default.
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