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- W2883233049 abstract "Background and Purpose— Previously, we showed that 17β-estradiol (E2) treatment prevented the subarachnoid hemorrhage (SAH)-induced cerebral vasospasm in male rats. The aim of this study was designed to further delineate the molecular mechanisms underlying E2-induced inhibition of inducible nitric oxide synthase (iNOS) upregulation and relief of vasospasm caused by SAH. Methods— The 2-hemorrhage SAH model was induced by 2 autologous injections of blood into the cisterna magna of adult male rats. The rats were then subcutaneously implanted of a Silastic tube containing corn oil with or without 17β-estradiol benzoate and received daily intraperitoneal injections of various doses of ICI 182,780, a nonselective estrogen receptor (ER) antagonist, for 7 days after the first hemorrhage. Basilar arteries were then removed for protein extraction, RNA isolation, and gel mobility assay. The protein levels of iNOS, p65, and ER were examined by Western blot analysis, and that iNOS mRNA expression was evaluated by reve..." @default.
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- W2883233049 date "2006-12-01" @default.
- W2883233049 modified "2023-09-26" @default.
- W2883233049 title "17β-Estradiol Inhibits Subarachnoid Hemorrhage–Induced Inducible Nitric Oxide Synthase Gene Expression by Interfering With the Nuclear Factor κB Transactivation" @default.
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