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- W2883662454 abstract "Abstract The current study investigated the use of two cationic peptides, Xentry-KALA (XK) and Xentry-Protamine (XP), for intracellular delivery of Connexin43 antisense oligonucleotides (Cx43AsODN). The charge and size of Cx43AsODN:XK and Cx43AsODN:XP complexes was determined by Zetasizer analysis. The earliest positive zeta potential reading was obtained at a 1:2 and 1:1.2 charge ratio of Cx43AsODN:XK and Cx43AsODN:XP respectively, with Cx43AsODN:XK resulting in overall larger complexes than Cx43AsODN:XP. Gel shift mobility assays revealed complete complex formation at a 1:2.5 and 1:2.2 charge ratio of Cx43AsODN:XK and Cx43AsODN:XP, respectively. Cellular uptake studies were carried out in ARPE-19 cells. While both complexes were able to enter the cells, Cx43AsODN:XK uptake appeared punctate and circular indicative of endosomal containment. Cx43AsODN:XP uptake, in contrast, resulted in diffuse appearance inside the cell suggesting endosomal escape of the cargo. Finally, western blot analysis confirmed that Cx43AsODN:XP was able to knockdown Cx43 expression in these cells under normal and hypoxic conditions." @default.
- W2883662454 created "2018-08-03" @default.
- W2883662454 creator A5020069021 @default.
- W2883662454 creator A5037010762 @default.
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- W2883662454 date "2018-07-26" @default.
- W2883662454 modified "2023-10-12" @default.
- W2883662454 title "Intracellular oligonucleotide delivery using the cell penetrating peptide Xentry" @default.
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- W2883662454 doi "https://doi.org/10.1038/s41598-018-29556-7" @default.
- W2883662454 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6062516" @default.
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- W2883662454 hasPublicationYear "2018" @default.
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