FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2883841194> ?p ?o ?g. }

• W2883841194 endingPage "91" @default.
• W2883841194 startingPage "84" @default.
• W2883841194 abstract "Protein purification is essential in the study of protein structure and function, and the development of novel therapeutics. Many studies require purifying multiple proteins at once, increasing the demand for improved purification methods. We hypothesized that multiple chromatography columns could be interfaced with a multi-well collection plate for rapid and convenient protein purification without the need of expensive instrumentation. As such, we developed a multi-column plate adapter (MCPA), which provides an economical yet versatile and time efficient, high-throughput protein purification system. The MCPA system simultaneously purified milligrams of different proteins under gravity or under vacuum for faster purification. The MCPA handles up to twenty-four 12 mL columns and multiple MCPA's in sequence allow milligram-scale purification of 96 different samples with relative ease. We also used the MCPA system for large scale affinity purification of four proteins, providing sufficient yields and purity for protein crystallization and biophysical characterization. The MCPA system is ideal for optimizing resin type and volume or any other purification parameter by customizing individual columns during the same purification. The high-throughput and versatile nature of this system should prove to be useful in obtaining adequate amounts of protein for subsequent analyses in any laboratory setting." @default.
• W2883841194 created "2018-08-03" @default.
• W2883841194 creator A5007756352 @default.
• W2883841194 creator A5016240689 @default.
• W2883841194 creator A5018834049 @default.
• W2883841194 creator A5051125069 @default.
• W2883841194 creator A5060663639 @default.
• W2883841194 creator A5081512444 @default.
• W2883841194 creator A5090090406 @default.
• W2883841194 date "2018-12-01" @default.
• W2883841194 modified "2023-10-07" @default.
• W2883841194 title "A multi-column plate adapter provides an economical and versatile high-throughput protein purification system" @default.
• W2883841194 cites W1588979652 @default.
• W2883841194 cites W1964778816 @default.
• W2883841194 cites W1975447903 @default.
• W2883841194 cites W1989703672 @default.
• W2883841194 cites W1994787582 @default.
• W2883841194 cites W2003449739 @default.
• W2883841194 cites W2006054867 @default.
• W2883841194 cites W2025665147 @default.
• W2883841194 cites W2029454612 @default.
• W2883841194 cites W2047995544 @default.
• W2883841194 cites W2050893923 @default.
• W2883841194 cites W2050957868 @default.
• W2883841194 cites W2058933664 @default.
• W2883841194 cites W2062992390 @default.
• W2883841194 cites W2067467942 @default.
• W2883841194 cites W2072404529 @default.
• W2883841194 cites W2084123824 @default.
• W2883841194 cites W2095373397 @default.
• W2883841194 cites W2103561755 @default.
• W2883841194 cites W2104072424 @default.
• W2883841194 cites W2104571730 @default.
• W2883841194 cites W2106246537 @default.
• W2883841194 cites W2111699871 @default.
• W2883841194 cites W2118122534 @default.
• W2883841194 cites W2131270941 @default.
• W2883841194 cites W2148939731 @default.
• W2883841194 cites W2206571153 @default.
• W2883841194 cites W2238146247 @default.
• W2883841194 cites W2238796322 @default.
• W2883841194 cites W2440461123 @default.
• W2883841194 cites W2498877559 @default.
• W2883841194 cites W2508767729 @default.
• W2883841194 cites W2565356656 @default.
• W2883841194 cites W2601563728 @default.
• W2883841194 cites W2736403501 @default.
• W2883841194 cites W2792119560 @default.
• W2883841194 cites W67246682 @default.
• W2883841194 doi "https://doi.org/10.1016/j.pep.2018.07.009" @default.
• W2883841194 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6147247" @default.
• W2883841194 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30041031" @default.
• W2883841194 hasPublicationYear "2018" @default.
• W2883841194 type Work @default.
• W2883841194 sameAs 2883841194 @default.
• W2883841194 citedByCount "7" @default.
• W2883841194 countsByYear W28838411942021 @default.
• W2883841194 countsByYear W28838411942022 @default.
• W2883841194 countsByYear W28838411942023 @default.
• W2883841194 crossrefType "journal-article" @default.
• W2883841194 hasAuthorship W2883841194A5007756352 @default.
• W2883841194 hasAuthorship W2883841194A5016240689 @default.
• W2883841194 hasAuthorship W2883841194A5018834049 @default.
• W2883841194 hasAuthorship W2883841194A5051125069 @default.
• W2883841194 hasAuthorship W2883841194A5060663639 @default.
• W2883841194 hasAuthorship W2883841194A5081512444 @default.
• W2883841194 hasAuthorship W2883841194A5090090406 @default.
• W2883841194 hasBestOaLocation W28838411942 @default.
• W2883841194 hasConcept C104317684 @default.
• W2883841194 hasConcept C161176658 @default.
• W2883841194 hasConcept C177284502 @default.
• W2883841194 hasConcept C179247698 @default.
• W2883841194 hasConcept C181199279 @default.
• W2883841194 hasConcept C185592680 @default.
• W2883841194 hasConcept C2780430158 @default.
• W2883841194 hasConcept C2780659323 @default.
• W2883841194 hasConcept C41008148 @default.
• W2883841194 hasConcept C43617362 @default.
• W2883841194 hasConcept C55493867 @default.
• W2883841194 hasConcept C6557445 @default.
• W2883841194 hasConcept C86803240 @default.
• W2883841194 hasConcept C8985478 @default.
• W2883841194 hasConcept C9390403 @default.
• W2883841194 hasConceptScore W2883841194C104317684 @default.
• W2883841194 hasConceptScore W2883841194C161176658 @default.
• W2883841194 hasConceptScore W2883841194C177284502 @default.
• W2883841194 hasConceptScore W2883841194C179247698 @default.
• W2883841194 hasConceptScore W2883841194C181199279 @default.
• W2883841194 hasConceptScore W2883841194C185592680 @default.
• W2883841194 hasConceptScore W2883841194C2780430158 @default.
• W2883841194 hasConceptScore W2883841194C2780659323 @default.
• W2883841194 hasConceptScore W2883841194C41008148 @default.
• W2883841194 hasConceptScore W2883841194C43617362 @default.
• W2883841194 hasConceptScore W2883841194C55493867 @default.
• W2883841194 hasConceptScore W2883841194C6557445 @default.
• W2883841194 hasConceptScore W2883841194C86803240 @default.
• W2883841194 hasConceptScore W2883841194C8985478 @default.
• W2883841194 hasConceptScore W2883841194C9390403 @default.

• next