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- W2883855800 abstract "Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation and viral transduction of pro/pre-B cells, cell transplantation, and phenotypic analysis of induced T cells. The procedure is reproducible and straightforward, providing an approach for generating induced T cells for translational research." @default.
- W2883855800 created "2018-08-03" @default.
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- W2883855800 date "2018-09-01" @default.
- W2883855800 modified "2023-10-16" @default.
- W2883855800 title "A protocol for generating induced T cells by reprogramming B cells in vivo" @default.
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- W2883855800 doi "https://doi.org/10.1016/j.cr.2018.05.001" @default.
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