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• W2883886013 abstract "Although Methanosarcinales are versatile concerning their methanogenic substrates, the ability of Methanosarcina thermophila to use carbon dioxide (CO 2 ) for catabolic and anabolic metabolism was not proven until now. Here, we show that M. thermophila used CO 2 to perform hydrogenotrophic methanogenesis in the presence as well as in the absence of methanol. During incubation with hydrogen, the methanogen utilized the substrates methanol and CO 2 consecutively, resulting in a biphasic methane production. Growth exclusively from CO 2 occurred slowly but reproducibly with concomitant production of biomass, verified by DNA quantification. Besides verification through multiple transfers into fresh medium, the identity of the culture was confirmed by 16s RNA sequencing, and the incorporation of carbon atoms from 13 CO 2 into 13 CH 4 molecules was measured to validate the obtained data. New insights into the physiology of M. thermophila can serve as reference for genomic analyses to link genes with metabolic features in uncultured organisms." @default.
• W2883886013 created "2018-08-03" @default.
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• W2883886013 date "2018-07-17" @default.
• W2883886013 modified "2023-10-12" @default.
• W2883886013 title "Hydrogenotrophic Methanogenesis and Autotrophic Growth of<i>Methanosarcina thermophila</i>" @default.
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• W2883886013 doi "https://doi.org/10.1155/2018/4712608" @default.
• W2883886013 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6079545" @default.
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