Matches in SemOpenAlex for { <https://semopenalex.org/work/W2883993420> ?p ?o ?g. }
- W2883993420 endingPage "1854" @default.
- W2883993420 startingPage "1837" @default.
- W2883993420 abstract "Zika virus (ZV) infects neural stem cells (NSCs) and causes quiescence in NSCs, reducing the pool of brain cells, leading to microcephaly. Despite conscientious efforts, the molecular mechanisms for ZV-mediated effects on NSCs lack clarity. This study aimed to explore the underlying mechanisms for ZV-mediated induction of quiescence in the primary cultures of human fetal neural stem cells (fNSCs). We demonstrate that expression of ZV envelope (E) protein displays maximum quiescence in human fNSCs by accumulating cells in the G0/G1 phase of the cell cycle as compared to other non-structural proteins, viz. NS2A, NS4A and NS4B. E protein induces immature differentiation by induction of pro-neuronal genes in proliferating fNSCs, induces apoptosis in differentiating fNSCs 3 days post differentiation, and disrupts migration of cells from differentiating neurospheres. In utero electroporation of mouse brain with E protein shows drastic downregulation of proliferating cells in ventricular and subventricular zone regions. Global microRNA sequencing suggests that E protein modulates miRNA circuitry. Among differentially expressed miRNAs, we found 14 upregulated and 11 downregulated miRNAs. Mir-204-3p and mir-1273g-3p directly regulate NOTCH2 and PAX3 expression, respectively, by binding to their 3′UTR. Bioinformatic analysis using GO analysis for the targets of differentially expressed miRNAs revealed enrichment of cell cycle and developmental processes. Furthermore, WNT, CCKR, PDGF, EGF, p53, and NOTCH signaling pathways were among the top enriched pathways. Thus, our study provides evidence for the involvement of ZV E protein and novel insights into the molecular mechanism through identification of miRNA circuitry." @default.
- W2883993420 created "2018-08-03" @default.
- W2883993420 creator A5007762370 @default.
- W2883993420 creator A5023728741 @default.
- W2883993420 creator A5038068549 @default.
- W2883993420 creator A5048661525 @default.
- W2883993420 creator A5051850649 @default.
- W2883993420 creator A5061633603 @default.
- W2883993420 creator A5078112130 @default.
- W2883993420 creator A5081582960 @default.
- W2883993420 date "2018-07-26" @default.
- W2883993420 modified "2023-10-16" @default.
- W2883993420 title "Zika virus E protein alters the properties of human fetal neural stem cells by modulating microRNA circuitry" @default.
- W2883993420 cites W1483477190 @default.
- W2883993420 cites W1545235721 @default.
- W2883993420 cites W1856318570 @default.
- W2883993420 cites W1887157179 @default.
- W2883993420 cites W1966931836 @default.
- W2883993420 cites W1975771868 @default.
- W2883993420 cites W1979045365 @default.
- W2883993420 cites W1989915490 @default.
- W2883993420 cites W1990056527 @default.
- W2883993420 cites W2001193776 @default.
- W2883993420 cites W2001781896 @default.
- W2883993420 cites W2020956236 @default.
- W2883993420 cites W2034299131 @default.
- W2883993420 cites W2042530028 @default.
- W2883993420 cites W204382415 @default.
- W2883993420 cites W2045742755 @default.
- W2883993420 cites W2048552348 @default.
- W2883993420 cites W2057436796 @default.
- W2883993420 cites W2063511140 @default.
- W2883993420 cites W2063842517 @default.
- W2883993420 cites W2064994363 @default.
- W2883993420 cites W2072810552 @default.
- W2883993420 cites W2078325825 @default.
- W2883993420 cites W2082401415 @default.
- W2883993420 cites W2087966784 @default.
- W2883993420 cites W2103750856 @default.
- W2883993420 cites W2109120219 @default.
- W2883993420 cites W2112785261 @default.
- W2883993420 cites W2122575917 @default.
- W2883993420 cites W2127183015 @default.
- W2883993420 cites W2129743419 @default.
- W2883993420 cites W2142287130 @default.
- W2883993420 cites W2142758749 @default.
- W2883993420 cites W2154861644 @default.
- W2883993420 cites W2171297629 @default.
- W2883993420 cites W2172658925 @default.
- W2883993420 cites W2178728544 @default.
- W2883993420 cites W2281227512 @default.
- W2883993420 cites W2289779888 @default.
- W2883993420 cites W2290264819 @default.
- W2883993420 cites W2292422784 @default.
- W2883993420 cites W2316180691 @default.
- W2883993420 cites W2327959547 @default.
- W2883993420 cites W2346484133 @default.
- W2883993420 cites W2356757232 @default.
- W2883993420 cites W2360131028 @default.
- W2883993420 cites W2372368841 @default.
- W2883993420 cites W2395380272 @default.
- W2883993420 cites W2402880302 @default.
- W2883993420 cites W2435874442 @default.
- W2883993420 cites W2466116628 @default.
- W2883993420 cites W2471802609 @default.
- W2883993420 cites W2507412157 @default.
- W2883993420 cites W2512421145 @default.
- W2883993420 cites W2518533905 @default.
- W2883993420 cites W2529918142 @default.
- W2883993420 cites W2588268036 @default.
- W2883993420 cites W2602095193 @default.
- W2883993420 cites W2755187147 @default.
- W2883993420 cites W2761481149 @default.
- W2883993420 cites W2762425132 @default.
- W2883993420 cites W2769240730 @default.
- W2883993420 cites W4253966395 @default.
- W2883993420 doi "https://doi.org/10.1038/s41418-018-0163-y" @default.
- W2883993420 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6180120" @default.
- W2883993420 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30050059" @default.
- W2883993420 hasPublicationYear "2018" @default.
- W2883993420 type Work @default.
- W2883993420 sameAs 2883993420 @default.
- W2883993420 citedByCount "35" @default.
- W2883993420 countsByYear W28839934202019 @default.
- W2883993420 countsByYear W28839934202020 @default.
- W2883993420 countsByYear W28839934202021 @default.
- W2883993420 countsByYear W28839934202022 @default.
- W2883993420 countsByYear W28839934202023 @default.
- W2883993420 crossrefType "journal-article" @default.
- W2883993420 hasAuthorship W2883993420A5007762370 @default.
- W2883993420 hasAuthorship W2883993420A5023728741 @default.
- W2883993420 hasAuthorship W2883993420A5038068549 @default.
- W2883993420 hasAuthorship W2883993420A5048661525 @default.
- W2883993420 hasAuthorship W2883993420A5051850649 @default.
- W2883993420 hasAuthorship W2883993420A5061633603 @default.
- W2883993420 hasAuthorship W2883993420A5078112130 @default.
- W2883993420 hasAuthorship W2883993420A5081582960 @default.
- W2883993420 hasBestOaLocation W28839934201 @default.