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- W2884078327 abstract "A wider application of minicircle (MC) vectors in gene therapy research depends critically on the ability to purify supercoiled (sc) MC from related miniplasmid (MP) and parental plasmid (PP) impurities. This protocol describes a purification strategy that combines the in vitro enzymatic relaxation of sc MP and PP impurities by a nicking endonuclease, and topoisomer separation and RNA clearance by hydrophobic interaction chromatography. The time required to follow the full protocol, from production to isolation of sc MC, is approximately 50 h. The process delivers sc MCs that are virtually free from MP, PP, RNA, and protein impurities." @default.
- W2884078327 created "2018-08-03" @default.
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- W2884078327 date "2018-08-01" @default.
- W2884078327 modified "2023-10-18" @default.
- W2884078327 title "Production and Purification of Supercoiled Minicircles by a Combination of <i>In Vitro</i> Endonuclease Nicking and Hydrophobic Interaction Chromatography" @default.
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- W2884078327 doi "https://doi.org/10.1089/hgtb.2018.046" @default.
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