FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2884293496> ?p ?o ?g. }

• W2884293496 endingPage "682" @default.
• W2884293496 startingPage "671" @default.
• W2884293496 abstract "L-Tyrosine which is one of the terminal metabolites of highly regulated aromatic amino-acid biosynthesis pathway in Escherichia coli is a precursor for synthesis of L-Dopa. In this study, we report over production of L-Dopa by enhancing expression of rate limiting isoenzyme of shikimate kinase (aroL), chorismate synthase (aroC), aromatic-amino-acid aminotransferase (tyrB) and 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) form a plasmid module harboring five enzymes under two inducible promoters converting shikimate to tyrosine. 4-hydroxyphenylacetate-3-hydrolase (hpaBC) which converts L-Tyrosine to L-Dopa was expressed constitutively from a separate plasmid module. Feedback deregulated expression of 3-Deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase (aroG*) replacing wild type aroG under its natural promoter led to enhancement of L-Dopa production. Deletion of transcriptional repressor tyrR and links to other competing pathways improved titers of L-Dopa. We focused on having a balanced flux by constitutive expression of pathway enzymes from plasmid constructs rather than achieving higher amounts of catalytic protein by induction. We observed glycerol when used as a carbon source for the final strain led to low acid production. The best performing strain led to decoupling of acid production and product formation in bioreactor. Fed batch analysis of the final strain led to 12.5 g/L of L-Dopa produced in bioreactor." @default.
• W2884293496 created "2018-08-03" @default.
• W2884293496 creator A5004621115 @default.
• W2884293496 creator A5031095573 @default.
• W2884293496 creator A5051938836 @default.
• W2884293496 creator A5074970912 @default.
• W2884293496 creator A5080857013 @default.
• W2884293496 date "2018-07-17" @default.
• W2884293496 modified "2023-09-27" @default.
• W2884293496 title "Metabolic engineering of<i>Escherichia coli</i>W3110 strain by incorporating genome-level modifications and synthetic plasmid modules to enhance L-Dopa production from glycerol" @default.
• W2884293496 cites W1599969036 @default.
• W2884293496 cites W1693303585 @default.
• W2884293496 cites W1837905731 @default.
• W2884293496 cites W1963861426 @default.
• W2884293496 cites W1966949519 @default.
• W2884293496 cites W1967500610 @default.
• W2884293496 cites W1988431706 @default.
• W2884293496 cites W2004235182 @default.
• W2884293496 cites W2004243610 @default.
• W2884293496 cites W2014361215 @default.
• W2884293496 cites W2026430354 @default.
• W2884293496 cites W2034233874 @default.
• W2884293496 cites W2046942167 @default.
• W2884293496 cites W2063903089 @default.
• W2884293496 cites W2068175602 @default.
• W2884293496 cites W2070057555 @default.
• W2884293496 cites W2086572408 @default.
• W2884293496 cites W2097775940 @default.
• W2884293496 cites W2098667036 @default.
• W2884293496 cites W2110039658 @default.
• W2884293496 cites W2133597156 @default.
• W2884293496 cites W2150187794 @default.
• W2884293496 cites W2166064969 @default.
• W2884293496 cites W2342427357 @default.
• W2884293496 cites W2462493218 @default.
• W2884293496 cites W2604662273 @default.
• W2884293496 cites W2605712730 @default.
• W2884293496 cites W2606376977 @default.
• W2884293496 cites W2619319449 @default.
• W2884293496 cites W2739904912 @default.
• W2884293496 cites W2767460545 @default.
• W2884293496 cites W4235639095 @default.
• W2884293496 cites W4251312575 @default.
• W2884293496 cites W4253281475 @default.
• W2884293496 doi "https://doi.org/10.1080/10826068.2018.1487851" @default.
• W2884293496 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30015557" @default.
• W2884293496 hasPublicationYear "2018" @default.
• W2884293496 type Work @default.
• W2884293496 sameAs 2884293496 @default.
• W2884293496 citedByCount "16" @default.
• W2884293496 countsByYear W28842934962019 @default.
• W2884293496 countsByYear W28842934962020 @default.
• W2884293496 countsByYear W28842934962021 @default.
• W2884293496 countsByYear W28842934962022 @default.
• W2884293496 countsByYear W28842934962023 @default.
• W2884293496 crossrefType "journal-article" @default.
• W2884293496 hasAuthorship W2884293496A5004621115 @default.
• W2884293496 hasAuthorship W2884293496A5031095573 @default.
• W2884293496 hasAuthorship W2884293496A5051938836 @default.
• W2884293496 hasAuthorship W2884293496A5074970912 @default.
• W2884293496 hasAuthorship W2884293496A5080857013 @default.
• W2884293496 hasConcept C104317684 @default.
• W2884293496 hasConcept C150194340 @default.
• W2884293496 hasConcept C158448853 @default.
• W2884293496 hasConcept C181199279 @default.
• W2884293496 hasConcept C185592680 @default.
• W2884293496 hasConcept C22744801 @default.
• W2884293496 hasConcept C2776165026 @default.
• W2884293496 hasConcept C2777992278 @default.
• W2884293496 hasConcept C2778255928 @default.
• W2884293496 hasConcept C2778910516 @default.
• W2884293496 hasConcept C2779574690 @default.
• W2884293496 hasConcept C547475151 @default.
• W2884293496 hasConcept C553450214 @default.
• W2884293496 hasConcept C55493867 @default.
• W2884293496 hasConcept C6350086 @default.
• W2884293496 hasConcept C86803240 @default.
• W2884293496 hasConceptScore W2884293496C104317684 @default.
• W2884293496 hasConceptScore W2884293496C150194340 @default.
• W2884293496 hasConceptScore W2884293496C158448853 @default.
• W2884293496 hasConceptScore W2884293496C181199279 @default.
• W2884293496 hasConceptScore W2884293496C185592680 @default.
• W2884293496 hasConceptScore W2884293496C22744801 @default.
• W2884293496 hasConceptScore W2884293496C2776165026 @default.
• W2884293496 hasConceptScore W2884293496C2777992278 @default.
• W2884293496 hasConceptScore W2884293496C2778255928 @default.
• W2884293496 hasConceptScore W2884293496C2778910516 @default.
• W2884293496 hasConceptScore W2884293496C2779574690 @default.
• W2884293496 hasConceptScore W2884293496C547475151 @default.
• W2884293496 hasConceptScore W2884293496C553450214 @default.
• W2884293496 hasConceptScore W2884293496C55493867 @default.
• W2884293496 hasConceptScore W2884293496C6350086 @default.
• W2884293496 hasConceptScore W2884293496C86803240 @default.
• W2884293496 hasIssue "8" @default.
• W2884293496 hasLocation W28842934961 @default.
• W2884293496 hasLocation W28842934962 @default.
• W2884293496 hasOpenAccess W2884293496 @default.
• W2884293496 hasPrimaryLocation W28842934961 @default.

• next