Matches in SemOpenAlex for { <https://semopenalex.org/work/W2884813831> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2884813831 endingPage "364" @default.
- W2884813831 startingPage "360" @default.
- W2884813831 abstract "Objective To observe the effects of cigarette smoke extract (CSE) on the proliferation and secretion of hydrogen peroxide (H2O2) in human lung fibroblasts (HLFs) induced by transforming growth faetor-β1(TGF-β1).Methods Cultured HLFs were divided into a normal group and a model group induced by TGF-β1 (5 ng/mL),then intervened with CSE at different concentrations (0% ,2.5% ,5%, 10%, respectively).Brdu ELISA assay was used to detect cell proliferation.H2O2release from cultured cells was assayed using a fluorimetric method.Cellular localization of H2O2 and expression of α-SMA were performed using a fluorescent-labeling strategy.Results TGF-β1 stimulated group showed positive expression of α-SMA, implying TGF-β1 had induced fibroblasts to differentiate into myofibroblasts.In TGF-β1 stimulated group,2.5% and 5% CSE promoted cell proliferation (P < 0.01 or 0.05), while 10% CSE inhibited cell proliferation (P < 0.01).In the normal group, both low and high concentration of CSE inhibited cell proliferation (P < 0.01 or P < 0.05), and the inhibition effect was dose-dependent.HLF induced by TGF-β1 generated low constitutive levels of extracellular H2O2 that was markedly enhanced by CSE treatment (P < 0.01).Pretreatment with DPI, an inhibitor of NADPH oxidase, abolished secretion of H2O2.Cellular localization of H2O2 by a fluorescent-labeling strategy demonstrated that extracellular secretion of H2O2 is specific to the myofibroblast.Conclusions Low concentration of CSE can promote myofibroblast proliferation, and markedly increase extracellular secretion of H2O2.CSE possibly take part in the development and progress of idiopathic pulmonary fibrosis by increasing oxidative stress." @default.
- W2884813831 created "2018-08-03" @default.
- W2884813831 creator A5024117637 @default.
- W2884813831 creator A5079883312 @default.
- W2884813831 date "2009-01-01" @default.
- W2884813831 modified "2023-09-27" @default.
- W2884813831 title "Effects of Cigarette Smoke Extract on the Proliferation and Secretion of Hydrogen Peroxide in Human Lung Fibroblasts Induced by Transforming Growth Factor-β1" @default.
- W2884813831 hasPublicationYear "2009" @default.
- W2884813831 type Work @default.
- W2884813831 sameAs 2884813831 @default.
- W2884813831 citedByCount "0" @default.
- W2884813831 crossrefType "journal-article" @default.
- W2884813831 hasAuthorship W2884813831A5024117637 @default.
- W2884813831 hasAuthorship W2884813831A5079883312 @default.
- W2884813831 hasConcept C118131993 @default.
- W2884813831 hasConcept C126322002 @default.
- W2884813831 hasConcept C1491633281 @default.
- W2884813831 hasConcept C153911025 @default.
- W2884813831 hasConcept C185592680 @default.
- W2884813831 hasConcept C207865475 @default.
- W2884813831 hasConcept C2780559512 @default.
- W2884813831 hasConcept C28406088 @default.
- W2884813831 hasConcept C49039625 @default.
- W2884813831 hasConcept C533411734 @default.
- W2884813831 hasConcept C55493867 @default.
- W2884813831 hasConcept C62112901 @default.
- W2884813831 hasConcept C71924100 @default.
- W2884813831 hasConcept C86803240 @default.
- W2884813831 hasConcept C95444343 @default.
- W2884813831 hasConceptScore W2884813831C118131993 @default.
- W2884813831 hasConceptScore W2884813831C126322002 @default.
- W2884813831 hasConceptScore W2884813831C1491633281 @default.
- W2884813831 hasConceptScore W2884813831C153911025 @default.
- W2884813831 hasConceptScore W2884813831C185592680 @default.
- W2884813831 hasConceptScore W2884813831C207865475 @default.
- W2884813831 hasConceptScore W2884813831C2780559512 @default.
- W2884813831 hasConceptScore W2884813831C28406088 @default.
- W2884813831 hasConceptScore W2884813831C49039625 @default.
- W2884813831 hasConceptScore W2884813831C533411734 @default.
- W2884813831 hasConceptScore W2884813831C55493867 @default.
- W2884813831 hasConceptScore W2884813831C62112901 @default.
- W2884813831 hasConceptScore W2884813831C71924100 @default.
- W2884813831 hasConceptScore W2884813831C86803240 @default.
- W2884813831 hasConceptScore W2884813831C95444343 @default.
- W2884813831 hasIssue "4" @default.
- W2884813831 hasLocation W28848138311 @default.
- W2884813831 hasOpenAccess W2884813831 @default.
- W2884813831 hasPrimaryLocation W28848138311 @default.
- W2884813831 hasRelatedWork W11259670 @default.
- W2884813831 hasRelatedWork W1984790811 @default.
- W2884813831 hasRelatedWork W2032197623 @default.
- W2884813831 hasRelatedWork W2040617911 @default.
- W2884813831 hasRelatedWork W2047317966 @default.
- W2884813831 hasRelatedWork W2109634521 @default.
- W2884813831 hasRelatedWork W2348883355 @default.
- W2884813831 hasRelatedWork W2363171631 @default.
- W2884813831 hasRelatedWork W2365647196 @default.
- W2884813831 hasRelatedWork W2367679120 @default.
- W2884813831 hasRelatedWork W2378414960 @default.
- W2884813831 hasRelatedWork W2380616929 @default.
- W2884813831 hasRelatedWork W2388812444 @default.
- W2884813831 hasRelatedWork W2417449372 @default.
- W2884813831 hasRelatedWork W2586751832 @default.
- W2884813831 hasRelatedWork W2775192146 @default.
- W2884813831 hasRelatedWork W2884863293 @default.
- W2884813831 hasRelatedWork W3029885133 @default.
- W2884813831 hasRelatedWork W3031603741 @default.
- W2884813831 hasRelatedWork W141699272 @default.
- W2884813831 hasVolume "8" @default.
- W2884813831 isParatext "false" @default.
- W2884813831 isRetracted "false" @default.
- W2884813831 magId "2884813831" @default.
- W2884813831 workType "article" @default.