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- W2885149102 abstract "Novel cell-based therapies, including optimization of the wound microenvironment via chemical or genetic means, hold promise to revolutionize the treatment of chronic limb ischemia and ischemic wounds. We examined the hypothesis that increasing expression in ischemic tissue of the endothelial adhesion molecule E-selectin by adeno-associated viral transduction would promote angiogenesis in a murine model of hindlimb ischemia. 12-week-old female C57BL/6 mice (N=10) underwent unilateral femoral artery ligation. After ligation, each mouse was injected in the ipsilateral semimebranosus muscle with 1x108 viral particles of either adeno-associated virus recombinant with human E-selectin gene (AAV/E-selectin) or adeno-associated virus recombinant with green fluorescent protein gene as control (AAV/GFP) suspended in phosphate-buffered saline (N=5/group). Ischemia was confirmed by laser Doppler perfusion imaging on post-ligation day one. Mice were sacrificed on post-ligation day seven and semimembranosus muscle har..." @default.
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- W2885149102 date "2015-05-01" @default.
- W2885149102 modified "2023-09-24" @default.
- W2885149102 title "Abstract 403: E-selectin Transfer by Recombinant Adeno-associated Virus Improves Angiogenesis in a Murine Model of Hindlimb Ischemia" @default.
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