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• W2885161502 endingPage "9079" @default.
• W2885161502 startingPage "9070" @default.
• W2885161502 abstract "In this study, α-galactooligosaccharides (α-GOSs) were synthesized using galactose as the substrate and α-galactosidase from Aspergillus niger as the catalyst. In the reaction, synthesized products of U1, U2, U3, and U4 were detected by high-performance liquid chromatography. By mass spectrometry, nuclear magnetic resonance, and 1-phenyl-3-methyl-5-pyrazolone derivatization, U1 was the mixture of disaccharides of α-d-Galp-(1→1)-α-d-Gal, α-d-Galp-(1→2)-α-d-Gal, α-d-Galp-(1→3)-α-d-Gal, α-d-Galp-(1→4)-α-d-Gal, U2 was identified to be α-d-Galp-(1→6)-α-d-Gal, U3 was the mixture of galactotrisaccharides linked by one α-(1→6)-glycosidic linkage and one other α-glycosidic linkage, and U4 was identified as α-d-Galp-(1→6)-α-d-Galp-(1→6)-α-d-Gal. Afterward, the synthesized α-GOSs (U1, U2, U3, U4, and their mixture) as well as α-GOSs (manninotriose, stachyose, ciceritol, and verbascose) obtained from natural materials were used as subjects to evaluate their immunomodulatory effects in vitro by culturing mouse macrophage RAW264.7 cells. The results showed that α-GOS with a higher degree of polymerization had better immunomodulatory activity, while to a certain extent, α-GOS linked with α-(1→6)-galactosidic linkage showed a better immunomodulatory effect." @default.
• W2885161502 created "2018-08-22" @default.
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• W2885161502 date "2018-08-07" @default.
• W2885161502 modified "2023-09-26" @default.
• W2885161502 title "Immunomodulatory Effects of Enzymatic-Synthesized α-Galactooligosaccharides and Evaluation of the Structure–Activity Relationship" @default.
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• W2885161502 doi "https://doi.org/10.1021/acs.jafc.8b01939" @default.
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• W2885161502 hasPublicationYear "2018" @default.
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