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- W2885163307 abstract "The use of CRISPR/Cas9 to knockout genes in zebrafish has been well established. However, to better model many human diseases that are caused by point mutations, a robust methodology for generating desirable DNA base changes is still needed. Recently, Cas9-linked cytidine deaminases (base editors) evolved as a strategy to introduce single base mutations in model organisms. They have been used to convert cytidine to thymine at specific genomic loci. Here we describe a protocol for using the base editing system in zebrafish and its application to reproduce a single base mutation observed in human Ablepharon-Macrostomia Syndrome." @default.
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- W2885163307 date "2018-11-01" @default.
- W2885163307 modified "2023-09-26" @default.
- W2885163307 title "Programmable base editing in zebrafish using a modified CRISPR-Cas9 system" @default.
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- W2885163307 doi "https://doi.org/10.1016/j.ymeth.2018.07.010" @default.
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