Matches in SemOpenAlex for { <https://semopenalex.org/work/W2885192969> ?p ?o ?g. }
- W2885192969 abstract "We previously described a mechanism of acquired resistance of B-cell acute lymphoblastic leukemia to CD19-directed chimeric antigen receptor T-cell (CART) immunotherapy. It was based on in-frame insertions in or skipping of CD19 exon 2. To distinguish between epitope loss and defects in surface localization, we used retroviral transduction and genome editing to generate cell lines expressing CD19 exon 2 variants (CD19ex2vs) bearing vesicular stomatitis virus G protein (VSVg) tags. These lines were negative by live-cell flow cytometry with an anti-VSVg antibody and resistant to killing by VSVg-directed antibody-drug conjugates (ADCs), suggestive of a defect in surface localization. Indeed, pulse-chase and α-mannosidase inhibitor assays showed that all CD19ex2vs acquired endoplasmic reticulum (ER)-specific high-mannose-type sugars but not complex-type glycans synthesized in the Golgi apparatus. When fused with green fluorescent protein (GFP), CD19ex2vs (including a mutant lacking the relevant disulfide bond) showed colocalization with ER markers, implying protein misfolding. Mass spectrometric profiling of CD19-interacting proteins demonstrated that CD19ex2vs fail to bind to the key tetraspanin CD81 and instead interact with ER-resident chaperones, such as calnexin, and ER transporters involved in antigen presentation. Thus, even the intact domains of CD19ex2vs cannot be easily targeted with ADCs or current CD19 CARTs but could serve as sources of peptides for major histocompatibility complex (MHC)-restricted presentation and T-cell receptor (TCR)-mediated killing." @default.
- W2885192969 created "2018-08-22" @default.
- W2885192969 creator A5004314956 @default.
- W2885192969 creator A5026678499 @default.
- W2885192969 creator A5038758227 @default.
- W2885192969 creator A5041931485 @default.
- W2885192969 creator A5058584806 @default.
- W2885192969 creator A5062942186 @default.
- W2885192969 creator A5065321275 @default.
- W2885192969 creator A5077440294 @default.
- W2885192969 creator A5077637690 @default.
- W2885192969 creator A5078077300 @default.
- W2885192969 creator A5082539381 @default.
- W2885192969 date "2018-11-01" @default.
- W2885192969 modified "2023-10-16" @default.
- W2885192969 title "CD19 Alterations Emerging after CD19-Directed Immunotherapy Cause Retention of the Misfolded Protein in the Endoplasmic Reticulum" @default.
- W2885192969 cites W1493013438 @default.
- W2885192969 cites W1572835322 @default.
- W2885192969 cites W1967512987 @default.
- W2885192969 cites W1977845897 @default.
- W2885192969 cites W1987134040 @default.
- W2885192969 cites W2003801319 @default.
- W2885192969 cites W2033032481 @default.
- W2885192969 cites W2033777508 @default.
- W2885192969 cites W2038426832 @default.
- W2885192969 cites W2041378699 @default.
- W2885192969 cites W2045017588 @default.
- W2885192969 cites W2062175368 @default.
- W2885192969 cites W2086080135 @default.
- W2885192969 cites W2086450075 @default.
- W2885192969 cites W2091789695 @default.
- W2885192969 cites W2092291840 @default.
- W2885192969 cites W2095614489 @default.
- W2885192969 cites W2105400883 @default.
- W2885192969 cites W2118241155 @default.
- W2885192969 cites W2118796952 @default.
- W2885192969 cites W2122550753 @default.
- W2885192969 cites W2126512002 @default.
- W2885192969 cites W2127013298 @default.
- W2885192969 cites W2130410032 @default.
- W2885192969 cites W2132207019 @default.
- W2885192969 cites W2143445112 @default.
- W2885192969 cites W2150351373 @default.
- W2885192969 cites W2169445756 @default.
- W2885192969 cites W2179454156 @default.
- W2885192969 cites W2241349587 @default.
- W2885192969 cites W2295039757 @default.
- W2885192969 cites W2320904042 @default.
- W2885192969 cites W2400439118 @default.
- W2885192969 cites W2496398835 @default.
- W2885192969 cites W2514418134 @default.
- W2885192969 cites W2539686048 @default.
- W2885192969 cites W2543564489 @default.
- W2885192969 cites W2560318964 @default.
- W2885192969 cites W2584459311 @default.
- W2885192969 cites W2599601523 @default.
- W2885192969 cites W2609835530 @default.
- W2885192969 cites W2768823259 @default.
- W2885192969 cites W2887326505 @default.
- W2885192969 doi "https://doi.org/10.1128/mcb.00383-18" @default.
- W2885192969 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6189457" @default.
- W2885192969 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30104252" @default.
- W2885192969 hasPublicationYear "2018" @default.
- W2885192969 type Work @default.
- W2885192969 sameAs 2885192969 @default.
- W2885192969 citedByCount "47" @default.
- W2885192969 countsByYear W28851929692018 @default.
- W2885192969 countsByYear W28851929692019 @default.
- W2885192969 countsByYear W28851929692020 @default.
- W2885192969 countsByYear W28851929692021 @default.
- W2885192969 countsByYear W28851929692022 @default.
- W2885192969 countsByYear W28851929692023 @default.
- W2885192969 crossrefType "journal-article" @default.
- W2885192969 hasAuthorship W2885192969A5004314956 @default.
- W2885192969 hasAuthorship W2885192969A5026678499 @default.
- W2885192969 hasAuthorship W2885192969A5038758227 @default.
- W2885192969 hasAuthorship W2885192969A5041931485 @default.
- W2885192969 hasAuthorship W2885192969A5058584806 @default.
- W2885192969 hasAuthorship W2885192969A5062942186 @default.
- W2885192969 hasAuthorship W2885192969A5065321275 @default.
- W2885192969 hasAuthorship W2885192969A5077440294 @default.
- W2885192969 hasAuthorship W2885192969A5077637690 @default.
- W2885192969 hasAuthorship W2885192969A5078077300 @default.
- W2885192969 hasAuthorship W2885192969A5082539381 @default.
- W2885192969 hasBestOaLocation W28851929691 @default.
- W2885192969 hasConcept C104317684 @default.
- W2885192969 hasConcept C123894998 @default.
- W2885192969 hasConcept C130361206 @default.
- W2885192969 hasConcept C147483822 @default.
- W2885192969 hasConcept C153911025 @default.
- W2885192969 hasConcept C158617107 @default.
- W2885192969 hasConcept C159047783 @default.
- W2885192969 hasConcept C195616568 @default.
- W2885192969 hasConcept C203014093 @default.
- W2885192969 hasConcept C2522874641 @default.
- W2885192969 hasConcept C2776408679 @default.
- W2885192969 hasConcept C2776507628 @default.
- W2885192969 hasConcept C40767141 @default.
- W2885192969 hasConcept C47450691 @default.
- W2885192969 hasConcept C55493867 @default.