Matches in SemOpenAlex for { <https://semopenalex.org/work/W2885203537> ?p ?o ?g. }
- W2885203537 abstract "Snake gourd seed lectin (SGSL) is a non-toxic homolog of type II ribosome-inactivating proteins (RIPs) which contain a catalytic domain and a lectin domain. Isothermal titration calorimetry (ITC) measurements of the interactions of the protein with LacNAc, Lac, Gal, Me-α-Gal were carried out and the crystal structures of the native protein and its complex with Lac were determined. The crystal structure of the Me-α-Gal complex has already been determined. While the crystal structure showed the presence of two-sugar-binding sites, one on each of the two domains of the lectin chain, ITC measurements indicated the presence of only one binding site. In order to resolve this anomaly, molecular dynamics (MD) simulations were carried out on the native protein and on its complexes with Me-α-Gal and Lac. Simulations were also performed on the protein after reducing the inter-chain disulfide bridge between the two chains. The crystal structures and the simulations confirmed the robustness of the protein structure, irrespective of the presence or absence of the disulfide bridge. The simulations indicated that although two sites can bind sugar, only the ligand at one site is retained in a dynamic situation. The studies thus bring out the subtle relationship between binding and retention of the ligand." @default.
- W2885203537 created "2018-08-22" @default.
- W2885203537 creator A5004276030 @default.
- W2885203537 creator A5018607234 @default.
- W2885203537 creator A5051985040 @default.
- W2885203537 creator A5067128501 @default.
- W2885203537 date "2018-08-06" @default.
- W2885203537 modified "2023-09-24" @default.
- W2885203537 title "Ligand binding and retention in snake gourd seed lectin (SGSL). A crystallographic, thermodynamic and molecular dynamics study" @default.
- W2885203537 cites W1482431053 @default.
- W2885203537 cites W1491809717 @default.
- W2885203537 cites W1563704931 @default.
- W2885203537 cites W1671256862 @default.
- W2885203537 cites W1671321627 @default.
- W2885203537 cites W1811324376 @default.
- W2885203537 cites W1976499671 @default.
- W2885203537 cites W1981021420 @default.
- W2885203537 cites W1983824250 @default.
- W2885203537 cites W1986191025 @default.
- W2885203537 cites W1991794210 @default.
- W2885203537 cites W1991874195 @default.
- W2885203537 cites W1996900288 @default.
- W2885203537 cites W1998889990 @default.
- W2885203537 cites W2012796753 @default.
- W2885203537 cites W2012861671 @default.
- W2885203537 cites W2014694459 @default.
- W2885203537 cites W2017196167 @default.
- W2885203537 cites W2019434845 @default.
- W2885203537 cites W2020357919 @default.
- W2885203537 cites W2026814795 @default.
- W2885203537 cites W2028492488 @default.
- W2885203537 cites W2031761311 @default.
- W2885203537 cites W2031901496 @default.
- W2885203537 cites W2036766805 @default.
- W2885203537 cites W2045683699 @default.
- W2885203537 cites W2049125285 @default.
- W2885203537 cites W2051628563 @default.
- W2885203537 cites W2055048897 @default.
- W2885203537 cites W2067174909 @default.
- W2885203537 cites W2078553720 @default.
- W2885203537 cites W2081693079 @default.
- W2885203537 cites W2082206582 @default.
- W2885203537 cites W2089207351 @default.
- W2885203537 cites W2095866399 @default.
- W2885203537 cites W2106778710 @default.
- W2885203537 cites W2108921801 @default.
- W2885203537 cites W2114814536 @default.
- W2885203537 cites W2120285043 @default.
- W2885203537 cites W2122109248 @default.
- W2885203537 cites W2128572087 @default.
- W2885203537 cites W2147949826 @default.
- W2885203537 cites W2153754702 @default.
- W2885203537 cites W2154263028 @default.
- W2885203537 cites W2154714625 @default.
- W2885203537 cites W2161605421 @default.
- W2885203537 cites W2163341755 @default.
- W2885203537 cites W2163481879 @default.
- W2885203537 cites W2165520485 @default.
- W2885203537 cites W2167029191 @default.
- W2885203537 cites W2168469272 @default.
- W2885203537 cites W2171268876 @default.
- W2885203537 cites W2171521392 @default.
- W2885203537 cites W2194696545 @default.
- W2885203537 cites W2322798020 @default.
- W2885203537 cites W2444633805 @default.
- W2885203537 cites W2615742986 @default.
- W2885203537 cites W2621631231 @default.
- W2885203537 cites W295780 @default.
- W2885203537 cites W4246412309 @default.
- W2885203537 cites W4252625568 @default.
- W2885203537 doi "https://doi.org/10.1093/glycob/cwy072" @default.
- W2885203537 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30099481" @default.
- W2885203537 hasPublicationYear "2018" @default.
- W2885203537 type Work @default.
- W2885203537 sameAs 2885203537 @default.
- W2885203537 citedByCount "1" @default.
- W2885203537 countsByYear W28852035372021 @default.
- W2885203537 crossrefType "journal-article" @default.
- W2885203537 hasAuthorship W2885203537A5004276030 @default.
- W2885203537 hasAuthorship W2885203537A5018607234 @default.
- W2885203537 hasAuthorship W2885203537A5051985040 @default.
- W2885203537 hasAuthorship W2885203537A5067128501 @default.
- W2885203537 hasBestOaLocation W28852035371 @default.
- W2885203537 hasConcept C116569031 @default.
- W2885203537 hasConcept C12554922 @default.
- W2885203537 hasConcept C147597530 @default.
- W2885203537 hasConcept C156911925 @default.
- W2885203537 hasConcept C170493617 @default.
- W2885203537 hasConcept C185592680 @default.
- W2885203537 hasConcept C2780758401 @default.
- W2885203537 hasConcept C55493867 @default.
- W2885203537 hasConcept C59593255 @default.
- W2885203537 hasConcept C8010536 @default.
- W2885203537 hasConcept C86803240 @default.
- W2885203537 hasConceptScore W2885203537C116569031 @default.
- W2885203537 hasConceptScore W2885203537C12554922 @default.
- W2885203537 hasConceptScore W2885203537C147597530 @default.
- W2885203537 hasConceptScore W2885203537C156911925 @default.
- W2885203537 hasConceptScore W2885203537C170493617 @default.
- W2885203537 hasConceptScore W2885203537C185592680 @default.