FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2885404885> ?p ?o ?g. }

• W2885404885 endingPage "3253" @default.
• W2885404885 startingPage "3253" @default.
• W2885404885 abstract "Abstract Droplet digital PCR (ddPCR) is a highly sensitive and rapid method for detecting mutant allele frequency (MAF). In preliminary work, our lower limit of detection for common myeloid gene mutations was 0.001% in peripheral blood and bone marrow compared to 0.1% with flow cytometry and 0.01% with real-time quantitative PCR, and turnaround time is 1 day. Furthermore, we detected leukemic mutant alleles in peripheral blood (PB), introducing the possibility of sparing painful bone marrow biopsy procedures to determine treatment response. Thus, we hypothesized that ddPCR is a feasible and accurate method for monitoring leukemic disease burden in PB for the prospective care of patients (pts) with AML. Eighteen patients (pts) with de novo, relapse/refractory, and secondary AML were recruited to an IRB-approved study (NCT02435550) and bone marrow (BM), peripheral blood (PB), and saliva were collected at standard clinical visits. Gene mutations were identified by whole-exome sequencing (WES) of BM specimens at study entry. For ddPCR interrogation, genomic DNA was isolated (Qiagen), and select primers and probes (Bio-Rad/IDT) were developed based on variants identified in WES data. Case-specific primers and probes were validated on archived specimens obtained at study entry. 12/18 pt mutanomes met criteria for primer/probe design. 8 pts are in the primer/probe design and validation stage and 4 have completed validation and serial analyses. WES identified, and ddPCR confirmed, at least 1 mutation per patient at the study entry timepoint. The mutations included NRAS G13R, NRAS G12A, CSF3R T618I, and IDH2 R172K. In 2 cases, we observed a reduction in both PB and saliva MAF that were consistent with the reduction in both BM and PB blasts after treatment, resulting in complete remissions. Although PB blasts were reduced in a third pt receiving ruxolitinib, the persistence of their CSF3R MAF in PB indicated a resistant AML clone. WES revealed the presence of NRAS G13R variant in a secondary AML pt; however, WES did not detect this NRAS G13R variant in a cryopreserved BM specimen obtained at the pts MDS diagnosis. Interestingly, ddPCR was able to detect NRAS G13R variant at 0.1% MAF in a PB sample obtained at MDS diagnosis, demonstrating the ultrasensitive detection of rare variants within a sample, and highlighting the subclonal evolution of this pt's malignancy. Rapid detection of myeloid-related somatic mutations in a variety of tissue sources (i.e., saliva, PB) will allow for noninvasive monitoring of AML tumor burden. ddPCR may be used to observe molecular response to treatment and to detect molecular residual disease and relapse prior to clinically indicated BM biopsies. Citation Format: Kimberly E. Hawkins, Cesia Salan, Madeleine Turcotte, Lauren T. Vaughn, Mei Zhang, Yanping Zhang, Barry Sawicki, Glenda G. Anderson, Nosha Farhadfar, Hemant S. Murthy, Biljana N. Horn, Helen L. Leather, Paul Castillo, Maxim Norkin, John W. Hiemenz, Randy A. Brown, William Slayton, Jack W. Hsu, John R. Wingard, Christopher R. Cogle, Leylah M. Drusbosky. Droplet digital PCR is a sensitive method for detecting refractory acute myeloid leukemia (AML) clones in peripheral blood and saliva [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 3253." @default.
• W2885404885 created "2018-08-22" @default.
• W2885404885 creator A5001898266 @default.
• W2885404885 creator A5008762363 @default.
• W2885404885 creator A5020415674 @default.
• W2885404885 creator A5024906743 @default.
• W2885404885 creator A5025323115 @default.
• W2885404885 creator A5028647693 @default.
• W2885404885 creator A5038067131 @default.
• W2885404885 creator A5042492175 @default.
• W2885404885 creator A5049745844 @default.
• W2885404885 creator A5051993838 @default.
• W2885404885 creator A5054420705 @default.
• W2885404885 creator A5065927929 @default.
• W2885404885 creator A5066770802 @default.
• W2885404885 creator A5068594394 @default.
• W2885404885 creator A5070373092 @default.
• W2885404885 creator A5071237407 @default.
• W2885404885 creator A5073484932 @default.
• W2885404885 creator A5078899007 @default.
• W2885404885 creator A5081032431 @default.
• W2885404885 creator A5085182402 @default.
• W2885404885 creator A5090486615 @default.
• W2885404885 date "2018-07-01" @default.
• W2885404885 modified "2023-09-27" @default.
• W2885404885 title "Abstract 3253: Droplet digital PCR is a sensitive method for detecting refractory acute myeloid leukemia (AML) clones in peripheral blood and saliva" @default.
• W2885404885 doi "https://doi.org/10.1158/1538-7445.am2018-3253" @default.
• W2885404885 hasPublicationYear "2018" @default.
• W2885404885 type Work @default.
• W2885404885 sameAs 2885404885 @default.
• W2885404885 citedByCount "1" @default.
• W2885404885 countsByYear W28854048852020 @default.
• W2885404885 crossrefType "journal-article" @default.
• W2885404885 hasAuthorship W2885404885A5001898266 @default.
• W2885404885 hasAuthorship W2885404885A5008762363 @default.
• W2885404885 hasAuthorship W2885404885A5020415674 @default.
• W2885404885 hasAuthorship W2885404885A5024906743 @default.
• W2885404885 hasAuthorship W2885404885A5025323115 @default.
• W2885404885 hasAuthorship W2885404885A5028647693 @default.
• W2885404885 hasAuthorship W2885404885A5038067131 @default.
• W2885404885 hasAuthorship W2885404885A5042492175 @default.
• W2885404885 hasAuthorship W2885404885A5049745844 @default.
• W2885404885 hasAuthorship W2885404885A5051993838 @default.
• W2885404885 hasAuthorship W2885404885A5054420705 @default.
• W2885404885 hasAuthorship W2885404885A5065927929 @default.
• W2885404885 hasAuthorship W2885404885A5066770802 @default.
• W2885404885 hasAuthorship W2885404885A5068594394 @default.
• W2885404885 hasAuthorship W2885404885A5070373092 @default.
• W2885404885 hasAuthorship W2885404885A5071237407 @default.
• W2885404885 hasAuthorship W2885404885A5073484932 @default.
• W2885404885 hasAuthorship W2885404885A5078899007 @default.
• W2885404885 hasAuthorship W2885404885A5081032431 @default.
• W2885404885 hasAuthorship W2885404885A5085182402 @default.
• W2885404885 hasAuthorship W2885404885A5090486615 @default.
• W2885404885 hasConcept C104317684 @default.
• W2885404885 hasConcept C121608353 @default.
• W2885404885 hasConcept C126322002 @default.
• W2885404885 hasConcept C178790620 @default.
• W2885404885 hasConcept C185592680 @default.
• W2885404885 hasConcept C21790070 @default.
• W2885404885 hasConcept C2777563447 @default.
• W2885404885 hasConcept C2778461978 @default.
• W2885404885 hasConcept C2778729363 @default.
• W2885404885 hasConcept C2780007613 @default.
• W2885404885 hasConcept C2781187634 @default.
• W2885404885 hasConcept C48126324 @default.
• W2885404885 hasConcept C49105822 @default.
• W2885404885 hasConcept C526805850 @default.
• W2885404885 hasConcept C54355233 @default.
• W2885404885 hasConcept C71924100 @default.
• W2885404885 hasConcept C86803240 @default.
• W2885404885 hasConceptScore W2885404885C104317684 @default.
• W2885404885 hasConceptScore W2885404885C121608353 @default.
• W2885404885 hasConceptScore W2885404885C126322002 @default.
• W2885404885 hasConceptScore W2885404885C178790620 @default.
• W2885404885 hasConceptScore W2885404885C185592680 @default.
• W2885404885 hasConceptScore W2885404885C21790070 @default.
• W2885404885 hasConceptScore W2885404885C2777563447 @default.
• W2885404885 hasConceptScore W2885404885C2778461978 @default.
• W2885404885 hasConceptScore W2885404885C2778729363 @default.
• W2885404885 hasConceptScore W2885404885C2780007613 @default.
• W2885404885 hasConceptScore W2885404885C2781187634 @default.
• W2885404885 hasConceptScore W2885404885C48126324 @default.
• W2885404885 hasConceptScore W2885404885C49105822 @default.
• W2885404885 hasConceptScore W2885404885C526805850 @default.
• W2885404885 hasConceptScore W2885404885C54355233 @default.
• W2885404885 hasConceptScore W2885404885C71924100 @default.
• W2885404885 hasConceptScore W2885404885C86803240 @default.
• W2885404885 hasIssue "13_Supplement" @default.
• W2885404885 hasLocation W28854048851 @default.
• W2885404885 hasOpenAccess W2885404885 @default.
• W2885404885 hasPrimaryLocation W28854048851 @default.
• W2885404885 hasRelatedWork W107128292 @default.
• W2885404885 hasRelatedWork W1975473919 @default.
• W2885404885 hasRelatedWork W1981236559 @default.
• W2885404885 hasRelatedWork W2090016234 @default.
• W2885404885 hasRelatedWork W2121159001 @default.
• W2885404885 hasRelatedWork W2347812052 @default.

• next