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- W2885512900 abstract "Abstract Non-human primates are our closest relatives and are of special interest for ecological, evolutionary and biomedical research. The Japanese macaque ( Macaca fuscata ) has contributed to the progress of primatology and neurosciences over 60 years. Despite this importance, the molecular and cellular basis of the Japanese macaque remains unexplored since useful cellular tools are lacking. Here we generated induced pluripotent stem cells (iPSCs) from skin fibroblasts of the Japanese macaque with Sendai virus or plasmid vectors. The Japanese macaque iPSCs (jm-iPSCs) were established under feeder-free culture conditions, but feeder cells turned out to be essential for their maintenance. The jm-iPSCs formed human iPSC-like flat colonies which were positive for pluripotent antigens including alkaline phosphatase, SSEA4, and TRA-1-81. They also expressed endogenous OCT3/4 , SOX2 , L-MYC , and KLF4 and other pluripotent marker genes. The potential to differentiate into all three germ layers and neural stem cells was confirmed by embryoid body and neurosphere formation, respectively. The jm-iPSCs will provide a robust in vitro tool for investigating the underlying mechanisms of development and physiology studies with the Japanese macaque." @default.
- W2885512900 created "2018-08-22" @default.
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- W2885512900 date "2018-08-15" @default.
- W2885512900 modified "2023-10-12" @default.
- W2885512900 title "Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata)" @default.
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- W2885512900 doi "https://doi.org/10.1038/s41598-018-30734-w" @default.
- W2885512900 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6093926" @default.
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