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• W2885557322 abstract "CRISPR activation (CRISPRa) system is the convenient tool for targeted-gene activation, it has been developed and combined with a lighting-based system that can control transcription initiation spatially and temporally by utilizing photoreceptor derived from plant Arabidopsis thaliana. A blue light photoreceptor the Cryptochrome 2 (CRY2), and its binding partner CIB1 will dimerize by exposure to the blue light and it has been applied to human cells. However, the application of a combination of these two systems to zebrafish cell is still not explored. We performed zebrafish gene activation using p65 and VP64 activators in the zebrafish cells (ZF4). Our study demonstrated that we have successfully controlled the transcription level of ASCL1a, BCL6a, and HSP70 genes using blue light-activated CRISPR activation system. The result showed that using this system, mRNA level expression of ASCL1a, BCL6a, and HSP70 genes increased after irradiated under blue light for several hours and significantly different to those which treated in the dark." @default.
• W2885557322 created "2018-08-22" @default.
• W2885557322 creator A5032598180 @default.
• W2885557322 creator A5071093979 @default.
• W2885557322 date "2018-11-01" @default.
• W2885557322 modified "2023-10-03" @default.
• W2885557322 title "Spatiotemporal control of zebrafish (Danio rerio) gene expression using a light-activated CRISPR activation system" @default.
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• W2885557322 doi "https://doi.org/10.1016/j.gene.2018.07.077" @default.
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