FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2885673719> ?p ?o ?g. }

• W2885673719 abstract "T cell-mediated immunotherapy of cancer has achieved remarkable results in hard-to-beat cancers. The main challenge of adoptive T-cell transfer (ACT) is its labor intensive and costly production and logistics as well as its dependency on the quality of the patient9s T cells. To overcome these hurdles we have designed a universal cell line for TCR expression by modifying the FDA-approved NK cell line, NK-92. Advantages of using this cell line is that it is easy to expand and can readily be genetically engineered. However, tumor cell recognition and killing by NK-92 is not antigen specific. This can be controlled by introducing an antigen receptor, such as a chimeric antigen receptor (CAR) or, as in the current work, a TCR. We herein present evidence that NK-92 can be modified to become a T cell-like lymphocyte which we named UK-92 cell (Universal Killer). UK-92 expressing a therapeutic TCR showed conserved binding capacity to the cognate pMHC. Phosphoflow cytometry results indicated that the introduced TCR was able to mediate intracellular signaling upon either crosslinking or cognate pMHC binding. Our data showed that both early and late TCR signalling players were activated in a TCR-specific manner (anti-CD3/anti-CD28 stimulation) and further in a pMHC specific manner. In vitro functional assays using TCRs isolated from both CD8 and CD4 T cells demonstrated that UK-92-TCR could be stimulated in a pMHC-specific manner and, importantly, could kill tumor cells specifically even in a tumor-like microenvironment such as spheroids. We have now shown in vitro that UK-92 cells are as specific and potent as redirected T cells to kill target cells. Finally, encouraging in vivo data showed that mice receiving UK92 cells expressing a therapeutic TCR experienced reduction in tumor load and enhanced survival compared with control mice. If confirmed, the use of UK92 as a universal cell line might pave the way to truly off-the-shelf therapeutic effector cells for cancer immunotherapy and leading to drastic reduction of cell production time, logistic and cost. Citation Format: Pierre Dillard, Nadia Mensali, June Helen Myklebust, Mickael Hebeisen, Gjertrud Skorstad, Marit Renee Myrhe, Anne Fane, Gustav Gaudernack, Gunnar Kvalheim, Else Marit Inderberg, Sebastien Walchli. A universal killer T-cell for adoptive cell therapy of cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 3586." @default.
• W2885673719 created "2018-08-22" @default.
• W2885673719 creator A5002818469 @default.
• W2885673719 creator A5003293427 @default.
• W2885673719 creator A5003977220 @default.
• W2885673719 creator A5004859097 @default.
• W2885673719 creator A5023792974 @default.
• W2885673719 creator A5024559548 @default.
• W2885673719 creator A5049072695 @default.
• W2885673719 creator A5059193770 @default.
• W2885673719 creator A5067305238 @default.
• W2885673719 creator A5076142358 @default.
• W2885673719 creator A5080684602 @default.
• W2885673719 date "2018-07-01" @default.
• W2885673719 modified "2023-09-25" @default.
• W2885673719 title "Abstract 3586: A universal killer T-cell for adoptive cell therapy of cancer" @default.
• W2885673719 doi "https://doi.org/10.1158/1538-7445.am2018-3586" @default.
• W2885673719 hasPublicationYear "2018" @default.
• W2885673719 type Work @default.
• W2885673719 sameAs 2885673719 @default.
• W2885673719 citedByCount "0" @default.
• W2885673719 crossrefType "proceedings-article" @default.
• W2885673719 hasAuthorship W2885673719A5002818469 @default.
• W2885673719 hasAuthorship W2885673719A5003293427 @default.
• W2885673719 hasAuthorship W2885673719A5003977220 @default.
• W2885673719 hasAuthorship W2885673719A5004859097 @default.
• W2885673719 hasAuthorship W2885673719A5023792974 @default.
• W2885673719 hasAuthorship W2885673719A5024559548 @default.
• W2885673719 hasAuthorship W2885673719A5049072695 @default.
• W2885673719 hasAuthorship W2885673719A5059193770 @default.
• W2885673719 hasAuthorship W2885673719A5067305238 @default.
• W2885673719 hasAuthorship W2885673719A5076142358 @default.
• W2885673719 hasAuthorship W2885673719A5080684602 @default.
• W2885673719 hasConcept C147483822 @default.
• W2885673719 hasConcept C148125776 @default.
• W2885673719 hasConcept C154317977 @default.
• W2885673719 hasConcept C167672396 @default.
• W2885673719 hasConcept C19317047 @default.
• W2885673719 hasConcept C202751555 @default.
• W2885673719 hasConcept C203014093 @default.
• W2885673719 hasConcept C2776090121 @default.
• W2885673719 hasConcept C2776107976 @default.
• W2885673719 hasConcept C3875195 @default.
• W2885673719 hasConcept C502942594 @default.
• W2885673719 hasConcept C55493867 @default.
• W2885673719 hasConcept C86803240 @default.
• W2885673719 hasConcept C8891405 @default.
• W2885673719 hasConcept C90375314 @default.
• W2885673719 hasConcept C95444343 @default.
• W2885673719 hasConceptScore W2885673719C147483822 @default.
• W2885673719 hasConceptScore W2885673719C148125776 @default.
• W2885673719 hasConceptScore W2885673719C154317977 @default.
• W2885673719 hasConceptScore W2885673719C167672396 @default.
• W2885673719 hasConceptScore W2885673719C19317047 @default.
• W2885673719 hasConceptScore W2885673719C202751555 @default.
• W2885673719 hasConceptScore W2885673719C203014093 @default.
• W2885673719 hasConceptScore W2885673719C2776090121 @default.
• W2885673719 hasConceptScore W2885673719C2776107976 @default.
• W2885673719 hasConceptScore W2885673719C3875195 @default.
• W2885673719 hasConceptScore W2885673719C502942594 @default.
• W2885673719 hasConceptScore W2885673719C55493867 @default.
• W2885673719 hasConceptScore W2885673719C86803240 @default.
• W2885673719 hasConceptScore W2885673719C8891405 @default.
• W2885673719 hasConceptScore W2885673719C90375314 @default.
• W2885673719 hasConceptScore W2885673719C95444343 @default.
• W2885673719 hasLocation W28856737191 @default.
• W2885673719 hasOpenAccess W2885673719 @default.
• W2885673719 hasPrimaryLocation W28856737191 @default.
• W2885673719 hasRelatedWork W2085723131 @default.
• W2885673719 hasRelatedWork W2153654458 @default.
• W2885673719 hasRelatedWork W2161402514 @default.
• W2885673719 hasRelatedWork W2885673719 @default.
• W2885673719 hasRelatedWork W2951042522 @default.
• W2885673719 hasRelatedWork W3046210616 @default.
• W2885673719 hasRelatedWork W3081061116 @default.
• W2885673719 hasRelatedWork W3142242060 @default.
• W2885673719 hasRelatedWork W4200582180 @default.
• W2885673719 hasRelatedWork W4224119345 @default.
• W2885673719 isParatext "false" @default.
• W2885673719 isRetracted "false" @default.
• W2885673719 magId "2885673719" @default.
• W2885673719 workType "article" @default.